2011, № 12

Karimov I.F., Manukhov I.V., Ivanov Y.B., Deryabin D.G. BACILLUS SUBTILIS VKPM V-10548 STRAIN AND IT APPLICATION FOR SERUM BLOOD BACTIRICIDAL ACTIVITY RESEARCHThe recombinant Bacillus subtilis strain carrying plasmid with gram-negative sea microorganism Photobacterium leiognathi luxAB genes, before each introduced gram-positive bacteria ribosome-binding site is designed. This strain characterized by high gene expression level of bacterial luciferase and was deposited in the Russian National Collection of Industrial Microorganisms under № V-10548. In researching of B.subtilis VKPM V-10548 reaction in contact with different component serum blood probes the correspondence between bioluminescence suppression and bactericidal effect level mainly determined by beta-lysine activity is shown. Key words: bioluminescence, lux-genes, Bacillus subtilis, bactericidal effect, beta-lysine

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References:

1. Cooper, E. L. Comparative immunology [Text] / E.L. Cooper // Curr. Pharm. Des. — 2003. — Vol. 9. — N 2. — P. 119–131.

2. Dunkelberger, J.R. Complement and its role in innate and adaptive immune responses [Text] / J.R. Dunkelberger, W.-C. Song // Cell Research. — 2010. — Vol. 20. — N 1. — P. 34-50.

3. Bukharin, O.V. Property and functions of plateletic cationic protein [Text] / O.V. Bukharin, K.G. Suleyimanov // Progress of modern biology. — 1998. — N 2. — P. 194-204.

4. Bukharin, O.V. System of beta-lysine and its role in clinical and experimental medicine [Text] / O.V. Bukharin, N.V. Vasilyev // Tomsk, Publisher TSU. — 1977. — 190 p.

5. Sarukhanov, V.Ya. Method of detection beta-lysin activity of agricultural animals blood [Text] / V.Ya. Sarukhanov, N.N. Isamov, P.G. Carin // Agricultural biology. — 2007. — N4. — P. 123–125.

6. Deryabin, D.G. Causes determining character of bacterial luminescence changing on serum blood influence [Text] / D.G. Deryabin, E.G. Polyakov // Microbiology. — 2005. — Vol. 74. — N 2. — P. 191–197.

7. Deryabin, D.G. On-line determination of serum bactericidal activity using recombinant luminescent bacteria [Text] / D.G. Deryabin, E.G. Polyakov // Bulletin of experimental biology and medicine — 2006. — N 8. — P. 200–204.

8. Lee, C.Y. The lux genes of the luminous bacterial symbiont, Photobacterium leiognathi, of the ponyfish. Nucleotide sequence, difference in gene organization, and high expression in mutant Escherichia coli [Text] / C.Y. Lee, R.B. Szittner, E.A. Meighen // Eur. J. Biochem. — 1991. — Vol.201. — N 1. — P. 161–167.

9. Francis, K.P. Monitoring bioluminescent Staphylococcus aureus infections in living mice using a novel luxABCDE construct [Text] / K.P. Francis, D. Joh, C. Bellinger-Kawahara, M.J. Hawkinson, T.F. Purchio, P.R. Contag // Infect. Immun. — 2000. — Vol. 68. — N 6. — P. 3594–3600.

10. Karimov, I.F. Peculiarities of luminescent response of Bacillus subtilis recombinant strain bearing cloned Vibro harveyi lux AB genes to the action of thermostable blood serum compounds [Text] / I.F. Karimov, I.V. Manukhov, V.Yu. Kotova, D.O. Omel'chenko, D.G. Deryabin // Biotechnology. — 2010. — N 2 — P. 25–30.

About this article

Authors: Ivanov Yu.B., Manuhov I.V., Karimov I.F., Deryabin D.G.

Year: 2011

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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