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Orenburg State University january 17, 2022   RU/EN
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Mushkova I.A., Borsenok S.A., Karimova A.N., Shkandina Y.V.
HISTOMORPHOLOGICAL CHANGES IN THE RABBIT CORNEA AFTER IMPLANTATION INTRACORNEAL LENSES AND ELECTRON MICROSCOPIC JUSTIFICATION OF THE RESULTS [№ 12 ' 2015]
Today on the international market is approved and available for clinical use different models of intracorneal lenses for surgical correction of presbyopia. Using the technical capabilities and production capacity of domestic manufacturers were modeled and manufactured lenses with geometric parameters, calculated using mathematical modeling taking into account characteristics of the cornea, made of modern polymeric materials. To study the biocompatibility of intracorneal lenses it is necessary to conduct preclinical studies of reactivity of cornea in response to the implantation of the developed products hydroxyethylmethacrylate (HEMA) and olygourethanmethacrylate (OUMA) in an experimental study in vivo and conduction electron-microscopic examination of intact "inlays". When carrying out scanning electron microscopy the surface and edges of intact lenses is rendered smooth surface with no visible chipping. In the study of the shape of the edge surface of intracorneal lenses from HEMA visualized jagged, irregular edge, and lenses from OUMA — smooth, with no visible chipping. When conducting in vivo confocal microscopy in the experimental groups was visualized single collagen fibers, activation of keratocytes, was determined hyperreflexivity and hypocellularity in the area of the lens, pseudokeratinization of the surface epithelium. In the group with the use of GEMA's lenses noted decrease of transparency of the extracellular matrix around the implant. During the light microscope in the experimental groups was visualized bed of inlay. Around bed in both groups was detected activation of cells, presumably native keratocytes. The epithelium and endothelium — no visible changes in the area of inlay and outside this zone. In the control group — no significant changes in all layers of the cornea. Thus, in the course of the study it was found that the developed products do not cause pronounced cellular and tissue response of the cornea of the rabbit, the rendered methods of in vivo confocal microscopy and the light microscopy. However, jagged, irregular shape of the edge surface of GEMA's lenses caused, apparently, more technically complicated manufacturing technology ("turning") can cause greater activation of cells around the bed of the cornea and a moderate seal of stroma around the lens, in comparison with OUMA's lenses.

Samkova E.B., Karimov I.F.
ASSESMENT OF LIGHTING SOURCES BIOSAFETY BY REPORTER LUMINESCENT BACTERIA [№ 6 ' 2015]
Abstract. In recent years a number of studies purpose was to develop a biosensor methods for the evaluation of mutagenic and carcinogenic effects of environmental factors on the cell, in particular biosensor test systems whole cells that have in their composition two main genetic elements: a promoter that responds to exposure to a toxicant, and gene-reporter transcribed from the promoter. It may be noted that reporter microorganisms very suitable object for the study of DNA-damaging effects, due to ease of cultivation, inexhaustible resources, to ease of changing the irradiation conditions, and finally the ability to transcriptional response that is easy to visualize the level of luminescence of the bacterial strain. In this work reporter luminescent strains that are based on E. coli K12 MG1655: E. coli K12 MG1655 recA::lux, E. coli K12 MG1655 colD::lux and E. coli K12 MG1655 ibpA::lux were used to assess the biosafety sources artificial light, with the first two luminescence respond to DNA damage, and the latter — on the damage of heat shock proteins. For creating optimal conditions for the work of strains was established method of estimating the light, and a comparison of the physical characteristics of light sources, including incandescent lamps, compact fluorescent lamps and LED lamps. The results allowed to state that all types of lamps are able to provide the DNA damaging effects in different degrees, but more important is the brand, rather than the type of lamp; wherein a correlation damage from ultraviolet radiation intensity emitted by all types of light sources. There was also a difference in the response of the features of the strains E. coli recA::lux and E. coli colD::lux.

Mushkova I.A., Semenov A.D., Sobolev N.P., Zakharova I.A., Karimova A.N., Maychuk N.V.
EVALUATION OF RESULTS FOR INDUCED AMETROPY REFRACTIVE CORRECTION IN PATIENTS AFTER RECONSTRUCTIVE SURGERY WITH IMPLANTATION IRIS-LENS DIAPHRAGM [№ 12 ' 2014]
The research is aimed to develop a diagnostic and treatment algorithm of choice kerato-refractive surgery after reconstructive surgery with implantation iris-lens diaphragm and evaluate the results of induced ametropy correction. We observed 41 eyes (41 patients) with residual ametropy after reconstructive surgery with implantation iris-lens diaphragm before and after keratorefractive operations. The developed algorithm of choice in patients with induced ametropy after reconstructive surgery with implantation iris-lens diaphragm, taking into account the data pachymetry, the localization of the scar and the state of anterior surface of the cornea, provides personalized comprehensive approach in determining the techniques of laser keratorefractive correction that allowed increasing uncorrected visual acuity in all cases. FemtoLASIK — effective and safe technologies allowing obtain high functional results in the correction of induced ametropia in patients after the reconstructive surgery with implantation iris-lens diaphragm.

Drozdova E.A., Karimov I.F.
RUMEN FLUID NORMAL COMPONENTS EFFECTS ON LUMINESCENCE LEVEL OF BIOSENSOR [№ 6 ' 2014]
The effect of real (native) and rumen fluid mixture model on the emission level of luminescent strain E.coli was considered. Revealed similar toxic effects studied biological fluids, developing in the first minutes of contact. Analyzed the impact of individual components on rumen fluid bacteria glow, as well as the contribution of individual components of the trophic substrate.

Bykovskaya L.V., Karimov D.R.
THE RESEARCH METHODS OF DIFFENRENTIALING AND INTEGRATING CIRCUITS [№ 9 ' 2013]
The were researched the methods of forming of short peaky impulses from square-wave impulses by the differentiating circuits and saw-edged impulses with the integrating circuits. The were displayed the physical and virtual experiments results. Use of virtual experiment for modeling of differentiating and integrating chains is proved.

Semenov A.D., Mushkova I.A., Karimova A.N.
COMPARATIVE EVALUATION OF THE RESULTS OF POSTKERATOPLASTY AMETROPY LASER CORRECTION [№ 4 ' 2013]
We observed and operated 29 patients with residual ametropy after penetrating keratoplasty: by FemtoLASIK technology — 11 eyes, by standard LASIK technology — 18 eyes. There was received an increase of uncorrected visual acuity from 0,12±0,1 to 0,51±0,23 and from 0,11±0,1 to 0,5±0,24, respectively. Comparative analysis of the results of laser correction showed that corneal flap formed by a femtosecond laser, is more uniformity and less deviation in thickness than a flap formed by microkeratome.

Karimova Z.Kh.
DYNAMICS OF VISUAL ACUITY AND VISUAL FIELD BORDERS IN PATIENTS WITH ANTERIOR ISCHEMIC NEYROOPTIKOPATIEY AFTER TREATMENT BIOMATERIALS ALLOPLANT [№ 12 ' 2012]
There has been estimated the dynamics of visual acuity and visual field in patients with anterior ischemic optic neuropathy after treatment with the use of Alloplant biomaterial.

Karimov I.F., Manukhov I.V., Ivanov Y.B., Deryabin D.G.
BACILLUS SUBTILIS VKPM V-10548 STRAIN AND IT APPLICATION FOR SERUM BLOOD BACTIRICIDAL ACTIVITY RESEARCH [№ 12 ' 2011]
The recombinant Bacillus subtilis strain carrying plasmid with gram-negative sea microorganism Photobacterium leiognathi luxAB genes, before each introduced gram-positive bacteria ribosome-binding site is designed. This strain characterized by high gene expression level of bacterial luciferase and was deposited in the Russian National Collection of Industrial Microorganisms under № V-10548. In researching of B.subtilis VKPM V-10548 reaction in contact with different component serum blood probes the correspondence between bioluminescence suppression and bactericidal effect level mainly determined by beta-lysine activity is shown.

Abramova L.M., Zhigunov O.Yu., Nasurdinova R.A., Karimova O.A.
SEED PRODUCTIVITY OF ATRAGENE SPECIOSA WEINM. IN NATURE POPULATIONS OF SOUTH URALS [№ 6 ' 2010]
Seed productivity of Atragene speciosa in 7 natural coenopopulations of mountain-wood zone and Trans-Urals of Bashkortostan Republic is studied. The productivity of given species high enough — from 40 to 68 pieces of seeds on 1 fruit, number of fruits reaches 15-17 pieces on 1 plant, the productivity factor makes from 0,52 to 0,90.

Abramova L.M., Karimova O.A., Lebedeva M.V.
EXPERIENCE OF CULTIVATING POLEMONIUM CAERULEUM L. IN FOREST-STEPPE ZONE OF BASHKORTOSTAN [№ 9 ' 2009]
The authors introduce results of an experiment of cultivating rare medical plant Polemonium caeruleum L. (the family Polеmoniaceae) in forest-steppe zone of Republic of Bashkortostan. They have studied phenology, biometry, seed productivity, germinating ability of seeds under the culture conditions. From 3 tested agrotechnical variants of cultivating Polemonium caeruleum the most appropriate is a variant with using ground seedlings with planting scheme 30х30 cm.

Karimov I.F., Ivanov Yu.B., Deryabin D.G.
INFLUENCE OF THROMBOCYTE CATION ALBUMEN ON BIOLUMINESCENCE AND VIABILITY OF RECOMBINANT STRAIN ESCHERICHIA COLI WITH CLONED LUX-OPERON PHOTOBACTERIUM LEIOGNATHI [№ 2 ' 2009]
High sensibility of bioluminescence response of recombinant strain E.coli K 12 TG1 with cloned luxCDABE with gene P.leiognathi 54D10 to the influence of thrombocyte cation albumen (TCA) is established in this article. Determined for TCA quantities IC50, corresponding to 50-percent inhibition of luminescence intensity from starting level form in the same system 3,3-7,1 mkg/ml. It is shown that concentration dependences of bioluminescence suppression at the presence of TCA are inferior a lot to the development true bactericidal effect regarding to used bacterial cell-targets. As one of the possible reasons of such phenomenon it was named direct abscopal influence of TCA on enzymatic system of luminescence generation.

Ignatenko Yu.V., Karimov A.G.
POVERTY OF THE RUSSIAN POPULATION AS A REASON DETERMINED CHILD NEGLECT [№ 1 ' 2008]
The problem of child neglect is researched as a social event, negative influenced on development and upbringing of growing up generation in this article. Social event of poverty as a factor caused existing of this event is regarded in this article with aim of objective conception about child neglect.

Deryabin D.G., Karimov I.F.
PECULIARITIES RECOMBINANT LUMINESCENT BACTERIUM REACTION WITH DIFFERENT LUX-OPERONS IN PHAGOCYTAL SYSTEM [№ 12 ' 2007]
Dependence of bioluminescent response of different recombinant strains Escherichia coli in phagocytal system from the nature of cloned lux-operons in them is determined in this article. It is shown that constructions’ using on the base of genetic material of sea luminescent bacterium Photobacterium leiognathi in interaction with neutrophils of peripheral human blood allows reaching direct proportionality between light intensity and evidence of killing effect developed in these conditions.

Deryabin D.G., Grudinin D.A., Karimov I.F.
BASING OF OPTIMAL REGIME OF RECOMBINANT LUMINESCENT BACTERIA’S OPSONIZATION [№ 12 ' 2006]
Optimal procedure of recombinant strain Escherichia coli K12 having luxCDABE gene P.leiognathi with using of normal human immunoglobulin in contrast to other potential opsonines (intact and thermoinactivated blood serum, complement) not giving bactericidal effect and minimal changed level of its initial bioluminescence concerning this microorganism is based in this article. It is shown that recombinant strains E.coli opsonizated with given way cause dose-dependent stimulation of luminol-dependent chemiluminescence of neutrophilic phagocytes but at the same contact themselves demonstrate progressive decrease of bioluminescence level the nature of which is determined admittedly with absorption and intracellular killing of bacterial cells.

Karimov R.Ya., Khazhin A.V.
TRAINING AND METHODS OF ACTIVE TEACHING IN THE SYSTEM OF STUDENTS’ UPBRINGING OF PHYSICAL TRAINING INSTITUTE [№ 10 ' 2006]
Some results of ethnocultural and communicative upbringing of future specialists of physical training are stated in this article. Theoretical and methodical aspects of anthropotechnical means and methods using at educational process of institute are presented here.


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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© Электронное периодическое издание: ВЕСТНИК ОГУ on-line (VESTNIK OSU on-line), ISSN on-line 1814-6465
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