Karimov I.F., Manukhov I.V., Ivanov Y.B., Deryabin D.G.
BACILLUS SUBTILIS VKPM V-10548 STRAIN AND IT APPLICATION FOR SERUM BLOOD BACTIRICIDAL ACTIVITY RESEARCH ^{[№ 12 ' 2011]}
The recombinant Bacillus subtilis strain carrying plasmid with gram-negative sea microorganism Photobacterium leiognathi luxAB genes, before each introduced gram-positive bacteria ribosome-binding site is designed. This strain characterized by high gene expression level of bacterial luciferase and was deposited in the Russian National Collection of Industrial Microorganisms under № V-10548. In researching of B.subtilis VKPM V-10548 reaction in contact with different component serum blood probes the correspondence between bioluminescence suppression and bactericidal effect level mainly determined by beta-lysine activity is shown.