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2011, № 12



Karimov I.F., Manukhov I.V., Ivanov Y.B., Deryabin D.G. BACILLUS SUBTILIS VKPM V-10548 STRAIN AND IT APPLICATION FOR SERUM BLOOD BACTIRICIDAL ACTIVITY RESEARCHThe recombinant Bacillus subtilis strain carrying plasmid with gram-negative sea microorganism Photobacterium leiognathi luxAB genes, before each introduced gram-positive bacteria ribosome-binding site is designed. This strain characterized by high gene expression level of bacterial luciferase and was deposited in the Russian National Collection of Industrial Microorganisms under № V-10548. In researching of B.subtilis VKPM V-10548 reaction in contact with different component serum blood probes the correspondence between bioluminescence suppression and bactericidal effect level mainly determined by beta-lysine activity is shown. Key words: bioluminescence, lux-genes, Bacillus subtilis, bactericidal effect, beta-lysine

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About this article

Authors: Ivanov Yu.B., Manuhov I.V., Karimov I.F., Deryabin D.G.

Year: 2011


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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