October 2015, № 10 (185)

Korotkovа A.M., Lebedev S.V., Rusakovа E.A. DNA-DAMAGING EFFECTS OF NANOPARTICLES NI° AND NIO FOR EXAMPLE A PLANT SPECIES TRITICUM VULGAREThe effects of spherical nanoparticles of nickel Ni˚ diameter of 70 ± 0,3 nm and nickel oxide NiO diameter of 94 ± 0,3 nm in three dilutions — 0.025, 0.05 and 0.1 M at the root of the 4-day seedlings of Triticum vulgare. After 48 hour incubation with nanoparticles plants recorded dose dependent increase in DNA fragmentation. Electrophoretic separation of DNA showed the change in mobility from the perspective of reducing the amount of content the least migratory agarose gel closest to the start of the fragments form I (1000 bp), constituting the largest amount in the control samples, and the transition of the bulk of the DNA fragments from -flowing nucleotides (form II — less than 1 000 bp). Thus, under the influence of NPs Ni° and NiO in concentrations 0.025–0.1 M there was an increase of less than 1 000 bp fragment (form II) to 53–59 % and 55–59,8 % with simultaneous reduction of form I at 16,6–20,9 % and 15,6–22 %, respectively, compared to the control. The calculated results for the coefficient electrophoretogram of DNA damage in the case of 0.025, 0.05 and 0.1 M nickel nanoparticles was 1.052 ± 0.009, 1.126 ± 0.043 and 1.2 ± 0.03, and nickel oxide nanoparticles — 0.982 ± 0.02, 1.053 ± 0.013 and 1.192 ± 0.21 (against the background of the control 0.398 ± 0.05), respectively. Linear DNA profile electrophoregrams testified that nanoparticles at low concentrations (0.025 and 0.05 M) cause system degradation to discrete DNA fragments from the nucleotide-flowing transition from the bulk of the DNA in the region of less than 1 000 bp and the concentration is 0.1 M appearance of apoptotic "steps" represented by a group of split peaks located descending area, in which a multiple number of DNA 180 bp.Key words: Triticum vulgare — nickel nanoparticles — reactive oxygen species — oxidative stress — the degradation of DNA.

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About this article

Authors: Korotkova A.M., Rusakova E.A., Lebedev S.V.

Year: 2015

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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