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October 2015, № 10 (185)



Davydova О.К., Аleshina Е.S., Zhilenkov А.V. USE OF THE LUMINESCING STRAINS OF MICROORGANISMS AT RESEARCH OF FULLERENES DERIVATIVES BIOLOGICAL ACTIVITY DEPENDENCE ON THEIR SURFACE CHARGE VALUEProduction of nanomaterials and their successful application in various spheres causes concern about their uncontrollable receipt in environment and, as a result, impacts on live organisms that raise a question of development of the express analysis of danger/safety of nanomaterials with use original sensory and the reporter test-systems on the basis of luminescent and non-luminescent microorganisms. In the presented work the biological (antimicrobial) activity of С60- and С70-fullerene derivatives is estimated and the mechanisms which are its cornerstone and determined by formation of physical contact with bacterial cell targets are discussed. The method of the bioluminescent analysis was used for determination of concentration of 12 originally synthesized С60- and С70-fullerene derivatives, causing suppression of bioluminescence by 60th minute of contact for 50 % of control values (EC50). It is shown that use of a sensory strain of Escherichia coli K12 TG1 allows to reveal antibacterial activity of a greater number of functionalized fullerene derivatives (at 6 of 12) and the level of sensitivity, than when using Bacillus subtilis B-10548 (at 3 of 12). Application of the modified gel electrophoresis method allowed to estimate the value and the size of electrophoretic mobility of the used С60- and С70-fullerene derivatives and a role of electrostatic interactions in their biological activity. On the basis of the received experimental results it can be concluded that the obtained data can be used for creation of the innovative nanodisinfectants, are of interest to practical use in medicine and veterinary science.Key words: bacterial biosensors, Escherichia coli, Bacillus subtilis, fullerene derivatives, biological activity.

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References:

1. Buzea С., Pacheco I.I., Robbie K. Nanomaterials and nanoparticles: Sources and toxicity // Biointerphases. — 2007. –V. 2. — №4. — P. MR17–MR71.

2. Terekhova V. A., Gladkova M. M. Engineered nanomaterials in soil: Problems in assessing their effect on living organisms // Eurasian Soil Science. — 2013. — V. 46. — №12. — P. 1203–1210.

3. Johnston H. J., Hutchison G. R., Christensen F. M., Aschberger K., Stone V. The biological mechanisms and physicochemical characteristics responsible for driving fullerene toxicity // Toxicol. Sci. — 2010. — V. 114. — №2. — Р. 162–182.

4. Medvedeva S.E., Tyulkova N.A., Kuznetsov A.M., Rodicheva E.K. Bioluminescent bioassays based on luminous bacteria // J. of Sib. Fed. Univ. Biol. — 2009. — V. 4. — №2. — P. 418–452.

5. Li Q., Mahendra S., Lyon D.Y., Brunet L., Liga M.V., Li D., Alvarez P.J.J. Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications // Water research. –2008. — V. 42. –P. 4591–4602.

6. Blickley T.M., McClellan-Green P. Toxicity of aqueous fullerene in adult and larval Fundulus heteroclitus // Environ Toxicol Chem. — 2008. — V. 27. — №9.– P. 1964–1971.

7. Henry T.B., Petersen E.J., Compton R.N. Aqueous fullerene aggregates (nC60) generate minimal reactive oxygen species and are of low toxicity in fish: a revision of previous reports // Curr Opin Biotechnol. — 2011. — V. 22. — №4. — Р. 533–537.

8. Tang Y.J., Ashcroft J.M., Chen D., Min G., Kim C.H., Murkhejee B. et al. Charge-associated effects of fullerene derivatives on microbial structural integrity and central metabolism // Nano Lett. — 2007. — V.7. — №3. — P. 754–760.

9. Deryabin D.G., Davydova O.K., Yankina Z.Zh., Vasilchenko A.S., Miroshnikov S.A., Kornev A.B., Ivanchikhina A.V., Troshin P.A. The activity of [60]fullerene derivatives bearing amine and carboxylic solubilizing groups against Escherichia coli: a comparative study // J. of Nanomaterials. — 2014 V. 2014. — 9 p.

10. Kubatova H., Zemanova E., Klouda K., Bilek K., Kadukova J. Evaluation of Effects of C60 fullerene and its derivatives on selected microorganisms // J. of Materials Science and Engineering B. — 2013. — V.3. — №7. — P. 409–417.

11. Huh A.J., Kwon Y.J. "Nanoantibiotics": A new paradigm for treating infectious diseases using nanomaterials in the antibiotics resistant era // J. of Cont. Release. — 2011. — V. 156. — P. 128–145.

12. Sorokina E.V., Yudina T.P., Bubnov I.A., Danilov V.S. Assessment of iron toxicity using a luminescent bacterial test with an Escherichia coli recombinant strain // Microbiology/ — 2013. — V. 42. — № 4. — P. 439–444.

13. Deryabin D.G., Aleshina E.S., Efremova L.V.Application of the inhibition of bacterial bioluminescence test for assessment of toxicity of carbon-based nanomaterials // Microbiology. — 2012. — V. 81. — № 4. — P. 492–497.

14. Aleshina E.S., Bolodurina I.P., Deryabin D.G., Kucherenko M.G. Сorrection of results of bioluminescenct analysis in view of optical properties of observed carbonic nanomaterials // Vestnik OGU. — 2010. — №6. — P. 123–128.

15. Hartnagel U., Balbinot D., Jux N., Hirsch A. Electrophoresis of electrostatically assembled fullerene–porphyrin conjugates // Org. Biomol. Chem. — 2006. — V. 4. — P. 1785–1795.


About this article

Authors: Aleshina E.S., Davydova O.K., Zhilenkov A.V.

Year: 2015


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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