Duskaev G.K., Drozdova E.A., Alyoshina E.S., Bezryadina A.S.
ESTIMATION OF IMPACT ON THE INTESTINAL MICROFLOR OF BIRDS OF SUBSTANCES WITH ANTIBIOTIC, PROBIOTIC AND ANTI-QUORUM SENSING EFFECTS ^{[№ 11 ' 2017]}
The search for ways to increase the productivity of farm animals has always been one of the most important areas of research. Poultry farming was no exception. The study of new possibilities for using preparations of both synthetic and vegetable origin, consideration of their composition and properties, as well as investigation of the effects exerted by them on the intestinal microflora, will make it possible to avoid possible deplorable consequences for both poultry and human beings. Substances such as probiotic Lactobifadol, Biovit antibiotic and oak bark extract, in various volumes have both an overwhelming and stimulating effect on the intestinal microflora of the bird. Extract of the oak bark had a serious depressing effect on the intestinal biota of the bird. There was an obvious relationship between the increase in the volume of the extract and the degree of suppression of the intestinal microflora. The use of the probiotic Lactobifadol stimulated the quantitative composition of the intestinal microflora of the bird, however, when the probiotic was combined with the oak bark extract, the number of microorganisms in the sample was reduced. The use of antibiotic Biovit contributed to a serious inhibition of the intestinal microbiota of the bird. But when studying the joint action of the drug with the extract of the oak cortex, partial inactivation of the bactericidal effects occurred, as evidenced by higher values of the colony forming units (CFU), relative to the single use of the antibiotic.

Аleshina Е.S., Drozdova E.A.
ESTIMATION THE BIOTOXICITY OF CARBON NANOTUBES USEBASEDLUMINESCENT MICROORGANISMS ^{[№ 10 ' 2015]}
Despite the fact that carbon nanomaterials (CNM) was opened thirty years ago, it is still expanding the range of their application, as well as the amount of research devoted to the determination of their biological effects. In the literature, are constantly emerging data about the possible adverse effects caused by the CNM, developing at different levels of the organization alive. Therefore, the study questions the potential risks of their use still seems paramount. A possibility to use for this purpose natural and recombinant strains of luminescent bacteria is very important. In this context, the aim of this work was to evaluate the biological effects of carbon nanomaterials represented 8 samples and includes both single-walled and multi-walled nanotubes and nanofibers, methods of bioluminescence assay using luminescent recombinant Escherichia coli strain K12 TG1 with cloned luxCDABE genes of marine luminous bacteria Photobacterium leiognathi 54D10 marketed in a lyophilized form and natural strains Photobacterium phosphoreum-17 677f (based on commercial test systems "Mikrobiosensor-17 677f"). In experiments on E.coli CNM for compounds dispersed in distilled water, followed by ultrasonic treatment with EC20 and EC50 values were calculated from 5 to 8 CNM. When used as Photobacterium phosphoreum EC20 values were established for 7 of 8, and an EC50 of 5 to 8 CNM. It was found that the natural luminescent strain Photobacterium phosphoreum in 17-677f characterized by severe and rapid response to the impact of CNM than recombinant strain of Escherichia coli K12 TG1. At the same time the details of the response to use the touch-term strains determines they are not mutually exclusive, but complementary use.

Davydova О.К., Аleshina Е.S., Zhilenkov А.V.
USE OF THE LUMINESCING STRAINS OF MICROORGANISMS AT RESEARCH OF FULLERENES DERIVATIVES BIOLOGICAL ACTIVITY DEPENDENCE ON THEIR SURFACE CHARGE VALUE ^{[№ 10 ' 2015]}
Production of nanomaterials and their successful application in various spheres causes concern about their uncontrollable receipt in environment and, as a result, impacts on live organisms that raise a question of development of the express analysis of danger/safety of nanomaterials with use original sensory and the reporter test-systems on the basis of luminescent and non-luminescent microorganisms. In the presented work the biological (antimicrobial) activity of С60- and С70-fullerene derivatives is estimated and the mechanisms which are its cornerstone and determined by formation of physical contact with bacterial cell targets are discussed. The method of the bioluminescent analysis was used for determination of concentration of 12 originally synthesized С60- and С70-fullerene derivatives, causing suppression of bioluminescence by 60th minute of contact for 50 % of control values (EC50). It is shown that use of a sensory strain of Escherichia coli K12 TG1 allows to reveal antibacterial activity of a greater number of functionalized fullerene derivatives (at 6 of 12) and the level of sensitivity, than when using Bacillus subtilis B-10548 (at 3 of 12). Application of the modified gel electrophoresis method allowed to estimate the value and the size of electrophoretic mobility of the used С60- and С70-fullerene derivatives and a role of electrostatic interactions in their biological activity. On the basis of the received experimental results it can be concluded that the obtained data can be used for creation of the innovative nanodisinfectants, are of interest to practical use in medicine and veterinary science.

Drozdova E.A., Аleshina Е.S.
ANTAGONISTIC ACTIVITY OF STRAINS PROBIOTIC PREPARATIONS AGAINST TEST ORGANISM SALMONELLA ENTERITIDIS AND EFFICIENCY OF THEIR JOINT USE WITH ANTIBIOTICS WAS DETECTED ^{[№ 10 ' 2015]}
The result of the experiments was determined by antibiotic resistance and antagonistic activity of the strains entering into probiotic preparations in respect of the test organism Salmonella enteritidis and their possible combined action with antibiotics. The experiment was set in conditions in vitro. Against the background of antibiotic therapy in the treatment of various infections develop microecological bowel disorders such as dysbiosis. For the treatment of the effects of the most studied direction at the present time is the use of probiotics — biological drugs based on representatives of the normal microflora of man. The feasibility of the simultaneous application of probiotics most used in clinical practice, antibiotics are currently the subject of discussion, as probiotic microorganisms are sensitive to many antimicrobial agents. In this regard, we have conducted the following studies and research to substantiate the effectiveness of the joint application of probiotics based nonsporeforming bacteria with antibiotics in the treatment of Salmonella infection in in vitro.

Aleshina E.S., Efremova L.V.
ANTIBACTERIAL ACTIVITY OF CARBON-BASED NANOMATERIALS, REVEALED WITH USE OF LUMINESCING BIOTESTS ^{[№ 10 ' 2013]}
Methodical approaches to carrying out biotesting of carbon-based nanomaterials (CBN) with use of luminescing biotests are developed. Use of similar approaches allowed to calculate probable biologically safe concentration of CBN in natural and production environments, and also to identify the CBN main properties conducting to development of toxic effect.

Aleshina E.S., Deryabin D.G., Efremova L.V.
INVESTIGATON OF CARBON-BASED NANOMATERIALS BIOLOGICAL ACTIVITY IN BACTERICIDITY TEST ON ESCHERICHIA COLI AND BACILLUS SUBTILIS ^{[№ 12 ' 2011]}
Influence of 10 carbon-based nanomaterials (CBNs): single- and multi-walled carbon nanotubes, nanofibres, С60-, С70-fullerenes, and amorphous carbon on viability of Escherichia coli K12 TG1 and Bacillus subtilis 168 is investigated. With use of named modeling microorganisms the CBNs bactericidal effect which progressed in time and depended on nanocarbon structural organization is shown. Comparison of test objects reaction shows B.subtilis little big sensitivity in comparison E.coli at high comparability of the biotesting results (r=0,927; P <0,005).

Aleshina E.S., Bolodurina I.P., Deryabin D.G., Kucherenko M.G.
СORRECTION OF RESULTS OF BIOLUMINESCENCT ANALYSIS IN VIEW OF OPTICAL PROPERTIES OF OBSERVED CARBONIC NANOMATERIALS ^{[№ 6 ' 2010]}
A mathematical tool has been developed aiming at correction of results of bioluminescent analysis in view of optical properties of the examined samples. With its assistance a specialized computer application was created to quantitatively evaluate bioluminescence of bacteria existing in faintly clear (colored and/or blurred) environment. Use of this application while evaluating biotoxicity of carbonic nanomaterials allowed eliminating distorting effect of optical properties of observed compounds on the results of carried out analysis.

Aleshina A.I.
TOLERANCE RESEARCH OF ORENBURG UNIVERSITY STUDENTS ^{[№ 10 ' 2007]}
The analysis of conducted research of tolerance level of linguist-translators, students of Orenburg State University is made in this article. The character of tolerance levels changing both as quantitative and qualitative is revealed. And also summary conclusions are made in this work.