Sviridova Т.G., Salnikova Е.V., Romanenko N.A., Kanygina O.N.
CONCENTRATION OF ORGANOCHLORINE PESTICIDES ON THE SURFACE OF C₁₈-HYBRID SILICA GEL FOR SAMPLE PREPARATION IN WATER QUALITY CONTROL METHODS ^{[№ 12 ' 2017]}
The solid-phase microconcentration of organochlorine pesticides (alpha-hexachlorocyclohexan (α-HСН), gamma-hexachlorocyclohexan (γ-HСН) and a 4.4-dichlordifeniltrikhloretan (4.4-DDT) on the surface of the sorbent C₁₈-hybrid silica gel, is an alternative to the classical method of preparing water samples for gas chromatographic analysis on the separation of two liquid phases. The need for a transition to solid-phase extraction of samples is dictated by the fact that this modern method significantly saves the sample preparation time, reduces the number of races of walking materials several times, and also avoids secondary contamination of samples. The degree of extraction of pesticides from water samples was analyzed in static and dynamic conditions. The conditions of sorption extraction were optimized by varying the type of desorbent solution and the contact time of the phases. As a result, it was shown that the nature of the desorbent had no significant effect on the time to achieve sorption equilibrium, but an increase in the extraction rates of organic pesticides with an increase in the volume fraction of acetone in n-hexane was observed. In addition, it was found that the degree of recovery of 4.4-DDT in all variants of the experiment was lower in comparison with α-HСН and γ-HСН in similar conditions. The use of dynamic mode with the use of the semi-automatic microsyringe SGE-Chromatec eVoL^XR made it possible to slightly increase the recovery of all organochlorine pesticides used in comparison with the corresponding experiments in the static regime. In general, the results confirm the possibility of switching to this method of preparation of water samples for analytical control over the content of the examined organochlorine pesticides in the samples, and the calculated characteristics of the extraction rates make it possible to carry out the studies without losing the quality of the analysis.

Romanenko N.A., Sviridova Т.G., Salnikova Е.V.
ADHESION OF CELLS OF ESCHERICHIA COLI K-12 ON A SURFACE OF THE ORGANOMODIFIED SORBENTS ON THE BASIS OF SILICA GEL ^{[№ 9 ' 2017]}
Adhesion of cells of microorganisms on the surface organomodification sorbents for microextraction when working with the solutions containing chemical and biological pollyutant is an obligatory link in a chain of in the chain of physico-chemical interactions in the quantitative analysis. At the same time questions of efficiency of definition of chemical analytes in such systems are often connected with deterioration in accuracy and reproducibility of an analytical method. It is defined first of all by features of a structure of sorbents — porosity, high surface area, existence and the structure of the imparted functional groups. So, by preparation of tests of water by method of solid-phase microextraction for gas chromatography definition of organochlorine pesticides (the alpha and scale of isomers of hexachlorocyclohexane (α–HCH and γ-HCH) and a 4.4-dichlorodiphenyltrichloroethane (4.4-DDT)), is possible adhesion cells of Escherichia coli K-12 which are contained in natural sources as on silica gels with the surface modified by C₁₈-hydrocarbonic groups, and on the classical granulated silica gel without modification. Besides, the question of efficiency of microextraction of pesticides from the model solutions containing a suspension of E. coli cells demands studying. As a result of a research the high connecting ability of silica gels in relation to microorganisms has been shown. Timed establishment of sorption balance didn’t exceed 30 minutes, and the coefficient of distribution of microorganisms V_s when using unmodified silica gel was higher, in comparison with that for silica gel with a surface, containing the imparted functional C₁₈ groups that demonstrates smaller adhesive ability of the last. At joint sorption of microorganisms and organochlorine pesticides the distribution coefficient significantly didn’t differ from that, calculated earlier. At the same time indicators of efficiency of extraction 4.4-DDT decreased against the background of minor change of extent of extraction of HCH isomers. Thus, the situation of joint sorption of the studied pesticides and cages of E. coli without loss for quality of the analysis is represented possible.

Romanenko O.V.
SPECIFICITY OF FOREIGN VOCABULARY OF THE GERMAN LANGUAGE SWISS VARIANT ON THE LEVEL OF WORD-FORMATION ^{[№ 5 ' 2010]}
This article is devoted to the important problem of specific characteristics studying of foreign vocabulary in the Swiss variant of the German literary language on the world-formation level. Formation models are regarded and their typical peculiarities revealed in the literary language at comparison of world-formation levels of both variants (Swiss and own German) are analyzed.

Romanenko N.A., Terekhova M.S., Deryabin
ALKYLXYBENZOLS INFLUENCE ON FUNCTIONAL ACTIVITY OF FC-FRAGMENTS OF ANTIBODIES ^{[№ 12 ' 2009]}
Ability of alkylxybenzols (AOB) change the functional activity of Fc-fragments of antibodies responsible for the initiation of the classical wais of complement activation is studied in the system of complementdependent hemolysis. It is determined that in such system C6-AOB and C12-AOB but not in C1-AOB and C3-AOB have their own haemolytic effect in high concentrations. At lower concentrations C6-AOB and C12-AOB influence on the kinetic characteristics of reaction of complementdependent hemolysis causing a time increase of lysis coming of 50% target cells without changing of lytic reaction speed.

Romanenko M.A.
PECULIARITIES OF EVIDENCES COLLECTING AT CASES OF CRIMINAL VIOLATIONS OF COPYRIGHT IN THE SPHERE OF SOFTWARE SUPPORT ^{[№ 3 ' 2008]}
Peculiarities of evidences collecting at cases of criminal violations of copyright in the sphere of software support with accounting of modern condition of technical means of replication are regarded in this article. General classifications of sources of software support copies distribution are given in this work.