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October 2017, № 9 (209)

Romanenko N.A., Sviridova Т.G., Salnikova Е.V. ADHESION OF CELLS OF ESCHERICHIA COLI K-12 ON A SURFACE OF THE ORGANOMODIFIED SORBENTS ON THE BASIS OF SILICA GELAdhesion of cells of microorganisms on the surface organomodification sorbents for microextraction when working with the solutions containing chemical and biological pollyutant is an obligatory link in a chain of in the chain of physico-chemical interactions in the quantitative analysis. At the same time questions of efficiency of definition of chemical analytes in such systems are often connected with deterioration in accuracy and reproducibility of an analytical method. It is defined first of all by features of a structure of sorbents — porosity, high surface area, existence and the structure of the imparted functional groups. So, by preparation of tests of water by method of solid-phase microextraction for gas chromatography definition of organochlorine pesticides (the alpha and scale of isomers of hexachlorocyclohexane (α–HCH and γ-HCH) and a 4.4-dichlorodiphenyltrichloroethane (4.4-DDT)), is possible adhesion cells of Escherichia coli K-12 which are contained in natural sources as on silica gels with the surface modified by C18-hydrocarbonic groups, and on the classical granulated silica gel without modification. Besides, the question of efficiency of microextraction of pesticides from the model solutions containing a suspension of E. coli cells demands studying. As a result of a research the high connecting ability of silica gels in relation to microorganisms has been shown. Timed establishment of sorption balance didn’t exceed 30 minutes, and the coefficient of distribution of microorganisms Vs when using unmodified silica gel was higher, in comparison with that for silica gel with a surface, containing the imparted functional C18 groups that demonstrates smaller adhesive ability of the last. At joint sorption of microorganisms and organochlorine pesticides the distribution coefficient significantly didn’t differ from that, calculated earlier. At the same time indicators of efficiency of extraction 4.4-DDT decreased against the background of minor change of extent of extraction of HCH isomers. Thus, the situation of joint sorption of the studied pesticides and cages of E. coli without loss for quality of the analysis is represented possible.Key words: organomodification silica gel, adhesion, Escherichia coli K-12, organochlorine pesticides, extraction.


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About this article

Authors: Salnikova E.V., Sviridova T.G., Romanenko N.A.

Year: 2017

Sergey Aleksandrovich

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