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Vasilchenko A.S., Yarullina D.R., Nikiyan H.N., Teslya A.V. MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF THE BACTERIA BACILLUS CEREUS AT VARIOUS STAGES OF THE LIFE CYCLEUsing atomic force microscopy (AFM) the life cycle of spore-forming Gram-positive organisms Bacillus cereus IP 5832 was investigated. The morphological and mechanical changes of single bacterial cells by their long-term culturing were described. It is shown decreasing the number of viable cells at the 5 day of cultivation and no viable cells in bacterial population at 15 day. In this case, the presence of spores which are morphologically differentiated forms of resting cells was revealed by AFM.Key words: atomic force microscopy, life cycle, mechanical properties, bacterial cells, long-term culturing.
References:
1. Rang C.U., Peng A.Y., Chao L. Report temporal dynamics of bacterial aging and rejuvenation // Current Biology. — 2011. — V. 21, №21. — P. 1813–1816.
2. Smolenceva O.A., Yarullina D.R., Ilyinskaya O.N. Induction of NO synthesis in lactobacilli under stress // Molecular Genetics, Microbiology and Virology. — 2012. — №1. — P. 25–29.
3. Rang C.U., Peng A.Y., Poon A.F., Chao L. Ageing in Escherichia coli requires damage by an extrinsic agent // Microbiology. — 2012. — V. 158. — P. 1553–1559.
4. Vasilchenko A.S., Nikiyan H.N., Deryabin D.G. Atomic force microscopy study of magainin 2 versus human platelet extract action on Escherichia coli and Bacillus cereus // Journal of Biological Research-Thessaloniki. — 2013. — V. 19. –P. 3–9.
5. Hertz, H. Uber die Beruhrung Fester Elastischer Korper (On the contact of elastic solids) // J. Reine Angew. — 1881. — №92. — Р. 156–171.
6. Brehm-Stecher, B.F. Single-Cell Microbiology: Tools, technologies, and applications // Microbiology and molecular biology reviews. — 2004. — V. 68, №3. — P. 538–559.
7. Nikiyan H., Vasilchenko A., Deryabin D. Humidity-dependent bacterial cells functional morphometry investigations using atomic force microscope // International journal of microbiology. — 2010. — doi:10.1155/2010/704170
8. Hayhurst E.J., Kailas L., Hobbs J.K., Foster S.J. Cell wall peptidoglycan architecture in Bacillus subtilis // PNAS. — 2008. — V. 105, №38. — P. 14603–14608
9. Finkel, S.E. Long-term survival during stationary phase: evolution and the GASP phenotype // Nature. — 2006. — V. 4. — P. 113–120.
10. Aronson A.I., Fitz-James P. Structure and morphogenesis of the bacterial spore coat // Bacteriol. Rev. — 1976. — V. 40. — P. 360–402.
11. Plomp M., Leighton T.J., Wheeler K.E., Malkin A.J. The high-resolution architecture and structural dynamics of Bacillus spores // Biophysical journal. — 2005. — V. 8, №1. — P. 603–608.
About this article
Authors: Nikiyan A.N., Vasilchenko A.S., Yarullina D.R., Teslya A.V.
Year: 2012
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Editor-in-chief |
Sergey Aleksandrovich MIROSHNIKOV |
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