Davydova О.К., Nikiyan Н.N.
1,3-DIHYDROXY-4-HEXYLRESORCINOL INCREASES BACTERIAL CELLS SENSITIVITY TO DAMAGING ACTION OF CARBON NANOMATERIALS [№ 7 ' 2017]
The relevance of disinfecting of objects of the environment is defined by need of interruption of the main ways of bacterial infections transfer. To achieve this goal, non-reagent (physical) and reagent (chemical) methods are currently used. At the same time the first ones generally include ultraviolet treatment, and the second ones —treatment with oxidants or specially selected chemical compounds. However, over time, microorganisms develop protective mechanisms for such damaging effects, so it seems possible to sensitize (increase the sensitivity) bacterial cells to already known disinfectants, and to develop new disinfectants that realize previously unknown mechanisms of antibacterial activity. In presented work increase in sensitivity of bacterial cells to damaging action of carbon nanomaterials (fullerenes and carbon nanotubes) by use of 1,3-dihydroxy-4-hexylbenzene is shown. Its action on bacterial cells significantly increases their surface affinity, strengthening of the subsequent influence of carbon nanodisinfectants and facilitating the removal of aggregates of bacterial cells and the nanocarbon particles acting on them from the media to be purified. The experimental studies form a basis for development of innovative nanodisinfectants and their compositions interesting for practical use in a disinfectology, sanitation and hygiene.
Nikiyan H.N., Tatlybaeva E.B.
ATOMIC FORCE MICROSCOPYUSING IN IDENTIFICATION OF SPECIFICALLY MARKED MOLECULES AND MICROORGANISM CELLS [№ 13 ' 2015]
Identification of biological molecules and microorganisms is an important problem of the microbiology which is traditionally solved by means of the marked antibodies. However in most cases the corresponding methods are based on detection of a significant amount of the interacting molecules and don't allow to find out their distribution on a surface. One of possible solutions consists in use of a modern method of atomic force microscopy. In current work the specific complexes formedbynanodimensionallabels, containing colloidal gold or amorphous carbon on a surface of bacterial cages of S. aureus and on a surface of plastic microwell immunological plateswere found and studied by means of atomic force microscopy. Morphological characteristics and the map of distribution of the revealed complexes on the studied surfaces were established. In experiments with tags sensitivity of a method was defined and comparison with the enzyme-linked immunoassay was carried out. The received results indicate high sensitivity of the offered method and certain advantages of carbon labels in comparison with gold labels, because of their easy detection and unambiguity in identification on the received images. Identification of microorganisms in model two-component biological systems was carried out and approaches to marking of bacterial cages in complex biological mixes was developed for the directed their allocation and the subsequent studying. Thus, use of a atomic force microscope in combination with nanodimensional carbon and gold labels, allows to realize a new way of identification of single molecules and microorganisms in complex biological mixes.
Nikiyan H.N., Tatlybaeva E.B.
ACHIEVEMENTS AND DEVELOPMENT PROSPECTS OF ATOMIC FORCE MICROSCOPY IN MICROBIOLOGY [№ 6 ' 2014]
In this review the possibilities of atomic force microscopy in solving of microbiological problems over the last decade are considered. The main attention is paid to the achievements and successes in the field of biodetection, virology and studying the factors of biogenic and abiogenic influence on bacterial cells. Presented results show the potential of atomic force microscopy in detection and identification of single cells and molecules, investigations of mechanical and morphological properties of bacterial cells in response to different influences, as well as the study of the intermolecular and intramolecular interactions.
Vasilchenko A.S., Yarullina D.R., Nikiyan H.N., Teslya A.V.
MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF THE BACTERIA BACILLUS CEREUS AT VARIOUS STAGES OF THE LIFE CYCLE [№ 10 ' 2012]
Using atomic force microscopy (AFM) the life cycle of spore-forming Gram-positive organisms Bacillus cereus IP 5832 was investigated. The morphological and mechanical changes of single bacterial cells by their long-term culturing were described. It is shown decreasing the number of viable cells at the 5 day of cultivation and no viable cells in bacterial population at 15 day. In this case, the presence of spores which are morphologically differentiated forms of resting cells was revealed by AFM.
Vasilchenko A.S., Nikiyan H.N., Deryabin D.G.
THE USE OF AGAROSE FILMS AS MODEL STRUCTURES FOR ATOMIC FORCE MICROSCOPE PREPARATION FOR FURTHER STUDY OF THE ELASTIC PROPERTIES OF BACTERIAL CELLS [№ 12 ' 2011]
Elastic properties of agarose films depending on the concentration and relative humidity of the samples environment were quantitatively characterized using atomic force microscopy in force spectroscopy mode. The results are discussed in the context of agarose films use as a test reference material to prepare an atomic force microscope for the further study of the mechanical properties of bacterial cells.
Nikiyan N.G., Yamansarin I.I.
OPERATIONAL MODEL OF THE ASYNCHRONOUS MOTORS RELIABILITY IN UNDERGROUND MINE [№ 4 ' 2011]
The operational model of electric motors reliability, taking into account the peculiarities of operating of an underground mine: dust, humidity and temperature of mine air, is proposed in this work. The model is based on observational data for machine failure from 2006 till 2010, using the methods of factorial experiment planning.
FROM MATHEMATICAL MODEL OF REAL ELECTRIC MACHINE TO ITS PERMISSIBLE LOAD [№ 1 ' 2006]
The mathematical model (MM) of the real electric machine (REM) distinguished from idealized machine by deflections of constructive, technological and operation origin was worked out. Consecution of MM creation on the example of real nonsynchronous machine is presented in this article. At that the third harmonic of magnetic field in air-gap, changes of steel magnetic characteristics under influence of technological factors, symmetry violation of magnetic and electric chain of a machine are taken into account here. Determination of permissible load of nonsynchronous engine, which has defects of operation origin equal to nominal meaning at stator current, is presented here. Key words: mathematical model, deflection, real electric machine, nonsynchronous machine, permissible load
Nikiyan N.G., Sankov D.P.
ABOUT THE OPPORTUNITY OF DIRECT MEASUREMENT OF CURRENT FREQUENCY IN ROTATING SHORT-CIRCUITED ROTOR`S CONTOUR OF THE ASYNCHRONOUS ENGINE [№ 10 ' 2005]
The estimation is made of existing methods of currents frequency measurement in a short-circuited contour of a rotor of the asynchronous engine. Researches are described of a magnetic field at an end face of a shaft by results of which frequency can be determined of a current in a contour.
Nikiyan N.G., Surkov D.V.
MASTERY AND RATING OF THE ELECTROMAGNETIC DIAGNOSTICS METHODS OF THE ROTOR ECCENTRICITY OF THE INDUCTION MOTORS [№ 2 ' 2005]
There is an experimental study of two electromagnetic diagnostics methods of the rotor eccentricity of the induction motors: 1) according to the meaning of the EMF search coils system; 2) according to the meaning of the stator winding Y- point potential. These methods are proved to possess the necessary sensitivity for a practical use. Having compared the methods they've arrived t a conclusion that the second one has more chances to be a success.
Davydova O.K., Nikiyan A.N., Deryabin D.G.
FORMING OF WELL-ORDERED PERMOLECULAR STRUCTURES OF DNA IN WATER SOLUTIONS IN PRESENCE OF ALKYLRESORCINOLS [№ 1 ' 2005]
The forming of well-ordered line permolecular structures of high-polymer DNA, happened in water solutions in presence of low-molecular ligands from the group of alkylresorcinols is dangerous. Consistent stages of similar process, beginning with creation of micellesimolar structures on detached filaments of DNA and finishing with their integration in cable structures, surrounded by single alkylresorcinol "cover", are visualized with using of atomic, power and scanning electron microscopy. The model of interaction between these molecules are suggested in this article on the base of represented results, and also earlier got data about changes of physical and chemical characteristics od DNA and alkylresorcinols.
Salikova S.P., Stadnikov A.A., Nikiyan A.N.
ATOMIC-POWER MICROSCOPE AS A NEW OPPORTUNITY OF CARDIOMYOCYTES STUDYING [№ 1 ' 2003]
The article considers the atomic-power microscope work main principles and its application results when studying rats' myocardium cells surface. Atomic-power method advantages are mentioned. Atomic-power method application allows not only to visualize the cell surface but to gain information on its different properties.
Letuta S.N., Nikiyan A.N., Davidova O.K.
RODOMINE STAINS AND DNA INTERACTION. [№ 4 ' 2001]
The article deals with the investigation of rodamine stains and DNA interaction by means of spectrophotometric methods and probe scanning microscopy. If was shown that rodamines interact with macromolecules by cooperative fixation with the external past of polymeric chain. As a result organic nanostructures (supramolecular systems) are formed on the polymer surface. The stain molecule quantify is estimated. The distribution of generated structures along the double DNA spiral and their fusion in larger conglomerates are investigated. The authors draw a conclusion about double spiral screening by means of stains and the nucleinic acids inactivation resulting in violations of their biological functions.
MATHEMATICAL MODELS OF THREE-PHASE ASYNCHRONOUS MACHINE IN VIEW OF TECHNOLOGICAL AND MAINTENANCE DEVIATIONS. [№ 1 ' 2000]
The existing determined mathematical model (MM) of the three-phase asynchronous machine (AM) is advanced with reference to low power AM by the registration of the third harmonic of a magnetic field in an air split, influence of technological and operation deviations on magnetic properties of cores, nonuniformity of an air split and asymmetry of a short-circuited rotor cage. On the basis of advanced determined MM probability MM is developed which can be directly used in designing and production of serial AM.
|Sergey Aleksandrovich |