|
|
|
Tereshchenko A.V., Belyy Yu.A., Takhchidi E.Kh., Novikov S.V., Maychuk N.In., Usanova, G.Yu. EXPERIMENTAL STUDY OF THE EFFECT OF A 0.1 % SOLUTION OF BENZALKONIUM CHLORIDE ON THE CONDITION OF THE CORNEA IN RABBITSIn order to create an experimental model of toxic corneal erosion in rabbits we have studied clinical and functional condition of the cornea (with use of confocal microscopy and quantitative calculation of the area of desquamated epithelium) and comparative assessment of the effect by instillation surface-active 0.1 % solution of benzalkonium chloride (experimental group) four times a day for 7 days, and saline solution (control group) on the rabbit cornea. It was shown that instillation of 0.1 % benzalkonium chloride for 7 days induced toxic corneal erosion, which is perspective for further development of new methods for treatment and prevention of this nosology.Key words: benzalkonium chloride, corneal erosion, cytotoxicity, confocal microscopy.
References:
1. Astahov S.Ju., Tkachenko N.V. Diagnostic capabilities of confocal microscopy in the study of surface structures of the eyeball. Ophthalmic statements. 2009; 2(1): 82 — 89.
2. Erichev V. P., Ambarcumjan K. G. Preservatives and secondary dry eye syndrome with long-term local medical therapy of primary open-angle glaucoma. Glaucoma. 2011; 2: 59-66.
3. Ibragimova D.I. The choice of the algorithm of treatment of various forms of lesions of the cornea in dry eye syndrome (experimental and clinical study): author. Diss...../ PhD, 2014.
4. Kachalina G.F., Mushkova I.A., Kishkin Ju.I., Majchuk N.V. et. al. Preparation of the ocular surface in patients with compensated primary open-angle glaucoma subsequent to the correction of refractive disorders. Actual problems of ophthalmology. 2014; 3: 142-144.
5. Kuroedov A.V., Ogorodnikova V.Ju. Beta-blockers in the treatment of primary open-angle glaucoma. The advantages and disadvantages. Review. Ophthalmology. 2008; 4: 4-17.
6. Kushnir V.N., Suracel L.A., Lupan V.D. Assessment of the quality of life of patients with primary open-angle glaucoma treated medically. A collection of ROOF. 2012; 1: 229-233.
7. Ljapunov N.A., PurtovA.V., Dunaj E.V Surfactant, colloidal micellar and antibacterial properties of some cationic antiseptics. Pharmacy. 2013; 4: 44-47.
8. Majchuk D.Ju. Erosion of the cornea: clinical forms, new methods of treatment. Clinical ophthalmology. 2004; 5(1): 17-20.
9. Onishhenko A.L., Lihacheva I.G., Plastinina S.L., Tkachev V.A. The reasons for the low compliance of patients with glaucoma and ways of its correction. Glaucoma. 2009; 4: 39-42.
10. Baudouin C. Detrimental effect of preservatives in eyedrops: implications for the treatment of glaucoma. Acta Ophthalmol. 2008; 86(7): 716–726.
11. De Saint Jean M., Brignole F., Bringuier A.F. Effects of benzalkonium chloride on growth and survival of Chang conjunctival cells. Invest. Ophthalmol. Vis. Sci. 1999; 40(3): 619-630.
12. Furrer P., Berger J., Mayer J.M., Gurny R.A. comparative study of the ocular tolerance of three timolol-based preparations: the influence of preservatives on ocular tolerance. J Fr Ophtalmol. 2001; 24: 13–19.
13. Labbe A., Pauly A., Liang H., Brignole-Baudouin F., et. al. Comparison of toxicological profiles of benzalkonium chloride and polyquaternium-1: an experimental study. J Ocul Pharmacol Ther 22: 267–278.
14. Pisella P.J., Fillacier K., Elena P.P., Elena P.P. et. al. Comparison of the effects of preserved and unpreserved formulations of timolol on the ocular surface of albino rabbits. Ophthalmic Res. 32: 3–8.
15. Shustermann D., Matovinovic E., Salmon A. Does Haber's Law Apply to Human Sensory Irritation? Inhal Toxicol. 2006; 18: 457–471.
16. The definition and classification of dry eye disease: report of the definition and classification subcommittee of the International Dry EyeWorkShop 2007. Ocul Surf. 2007; 5: 75–92.
17. Xiong С., Chen D., Liu J., Liu B. et. al. A Rabbit Dry Eye Model Induced by Topical Medication of a Preservative Benzalkonium Chloride. Invest Ophthalmol Vis Sci. 2008; 49: 1850–1856.
18. Zhirong Lin., Xiaochen Liu.,Tong Zhou.,Yihui Wang. et. al. A mouse dry eye model induced by topical administration of benzalkonium chloride. Mol Vis. 2011; 25(17): 257–264.
About this article
Authors: Maychuk N.V., Tahchidi E.H., Usanova G.Yu., Tereshchenko A.V., Belyy Yu.A., Novikov S.V.
Year: 2015
|
|
Editor-in-chief |
Sergey Aleksandrovich MIROSHNIKOV |
|
|