2015, № 12 (187)

Volkova N.V., Iureva T.N. RESEARCH OF CYTOKINES BALANCE IN PATIENTS AFTER FISTULIZING ANTIGLAUCOMATOUS SURGERYEffect of fistulazing surgery in glaucoma is reduced in every third case in 1–3 years after the intervention. Prevention of inappropriate inflammatory reaction and an attempt to "control" the healing process allow to prolong the hypotensive effect and increase the effectiveness of these interventions. Understanding of the course of postoperative regenerative-reparative process and knowledge of the factors that determine the duration and sequence of its phases at both the local and systemic levels, these approaches do pathogenetically justified. The study of the lacrimal fluid by enzyme immunoassay with determination of the level of interleukin-8 (IL-8), interleukin-6 (IL-6), and isoforms of 121 and 165 of family of vascular endothelial growth factor VEGF-A. The patients (n = 42) with uncompensated glaucoma were examined. The control group included healthy subjects (n = 18). The dynamics of pro-inflammatory and pro-angiogenic cytokines in terms of the day before, on the 3^rd day and after 2 months after surgery was examined. The level of interleukin-8 was (656,8 ± 205,7) / (656,8 ± 205,7) / (175 ± 89,3) pg/ml. Interleukin-6 — (23,28 ± 9,03) / (47,7 ± 25) / (33,0 ± 92) pg/ml. Level of 121 and 165 isoforms of the VEGF family A — (703,8 ± 379,4) / (364,4 ± 150) / (590 ± 230,7) pg/ml (quantitative values are in the order of timeline). In the group of patients receiving prolonged scheme of anti-inflammatory therapy, with the inclusion Restasis ®, the final level of IOP was significantly lower (p < 0.05), as well as the number of patients with a successful outcome of the surgery. Dynamics of proinflammatory cytokines IL-8 and IL-6 indicates the initial changed local tissue reactivity and confirms the course of inflammatory response during for at least 2 months of the postoperative period. Dynamics of isoforms 121 and 165 of the VEGF family confirms long-term nature of processes of fibroplasy and vasculogenesis and justifies the possible inclusion of angiogenesis inhibitors in postoperative rehabilitation of patients. Determination of cytokine balance of lacrimal fluid justifies the schema of postoperative rehabilitation of patients after fistulizing antiglaucomatous surgery. The dynamics of pro-inflammatory IL-8 and IL-6 interleukins indicates the initial changes in local tissue reactivity and confirmed the inflammatory response during for at least 2 months of the postoperative period. Dynamics of isoforms 121 and 165 of the VEGF family confirms long-term nature of processes of fibroplasy and vasculogenesis and justifies the possible inclusion of angiogenesis inhibitors in postoperative rehabilitation of patients.Key words: cytokines of lacrimal fluid, scarring, fistulizing antiglaucomatous surgery, Cyclosporin A (Restasis).

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About this article

Authors: Yuryeva T.N., Volkova N.V.

Year: 2015

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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