October 2015, № 10 (185)

Barysheva E.S., Obedkova Y.A. DEVELOPMENT GENOMIC METHODS TO DETERMINE THE AUTHENTICITY OF FOODS OF ANIMAL AND VEGETABLE ORIGIN BASED SPECIFIC BIOLOGICAL MACROMOLECULESThe objective of this study was to develop a method for genomic confirmation of authenticity of food products and quality of raw materials used, based on the determination of specific biological molecules, in particular genomic DNA. Monitoring of this parameter is allowed, to make an indirect assessment of the quality of raw materials used for the manufacture of food products. The structures (DNA) are the natural biological objects, as well as the human body, which gives grounds to speak about the greater reliability of the genomic method to verify the authenticity of food products and identify the raw materials used. Thus, the genomic DNA contained in the test foods, served as an indicator substance that is initially present in the product and, therefore, no need to add it in the course of research that will reduce the cost and increase the reliability of the analysis results of the study. Objective: to put into practice the methodology for the analysis of genomic DNA damage degree of animal and vegetable origin, in order to confirm their authenticity. The object of the study were products of vegetable or animal origin, in combination: a product in the "native" state and its derivatives. The work performed in the laboratory, Department of Biochemistry and Molecular Biology, using techniques horizontal eletroforeza DNA by agarose gel electrophoresis, followed by visualization transilluminator manufactured by Vilber Lourmat (France), at a wavelength of 254 nm and processing the received data in the program ImageJ (developer UnitedStates Department of Healthand Human Services, version 1.45, 2011). Article 5 is set out on pages using the 2 figures.Key words: authenticity, genomic DNA, foodstuffs of plant and animal health.

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About this article

Authors: Obъedkova Yu.A., Barysheva E.S.

Year: 2015

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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