October 2015, № 10 (185)

Аleshina Е.S., Drozdova E.A. ESTIMATION THE BIOTOXICITY OF CARBON NANOTUBES USEBASEDLUMINESCENT MICROORGANISMSDespite the fact that carbon nanomaterials (CNM) was opened thirty years ago, it is still expanding the range of their application, as well as the amount of research devoted to the determination of their biological effects. In the literature, are constantly emerging data about the possible adverse effects caused by the CNM, developing at different levels of the organization alive. Therefore, the study questions the potential risks of their use still seems paramount. A possibility to use for this purpose natural and recombinant strains of luminescent bacteria is very important. In this context, the aim of this work was to evaluate the biological effects of carbon nanomaterials represented 8 samples and includes both single-walled and multi-walled nanotubes and nanofibers, methods of bioluminescence assay using luminescent recombinant Escherichia coli strain K12 TG1 with cloned luxCDABE genes of marine luminous bacteria Photobacterium leiognathi 54D10 marketed in a lyophilized form and natural strains Photobacterium phosphoreum-17 677f (based on commercial test systems "Mikrobiosensor-17 677f"). In experiments on E.coli CNM for compounds dispersed in distilled water, followed by ultrasonic treatment with EC20 and EC50 values were calculated from 5 to 8 CNM. When used as Photobacterium phosphoreum EC20 values were established for 7 of 8, and an EC50 of 5 to 8 CNM. It was found that the natural luminescent strain Photobacterium phosphoreum in 17-677f characterized by severe and rapid response to the impact of CNM than recombinant strain of Escherichia coli K12 TG1. At the same time the details of the response to use the touch-term strains determines they are not mutually exclusive, but complementary use.Key words: luminescent microorganism, biotoxicity, carbon nanotube.

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About this article

Authors: Aleshina E.S., Drozdova E.A.

Year: 2015

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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