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June 2015, № 6 (181)



Korotkova A.M., Davydova O.K. EFFECTS OF SYNTHETIC ALKYLRESORCINOLS ON DNA TOPOLOGICAL CHANGES, MEDIATED BY REACTIVE OXYGEN SPECIES IN VITRO SYSTEMThe problem of live systems protection against an oxidizing stress by means of various low-molecular organic compounds capable to neutralize reactive oxygen species attracts the growing interest in search, study and prospects of practical use of both naturaland synthetic antioxidants. Alkylresorcinols known by the function of adaptogens at plants and microorganisms can be also included to their number. In turn, a well-documented effect of alkylresorcinols concerning biopolymers and cells is change of their functional activity and resistance to extreme influences. Because the genetic structure is a one of the most vulnerable targets of various destructive factors, including oxidizing stressinductors, the aim of the work is experimental justification of a role of synthetic alkylresorcinols differing in chemical structure in protection of DNA against reactive oxygen species. The effects of destruction of topologically various molecules of pUC19 plasmid DNA associated with formation of single-stranded breaks and which are expressed in change of a ratio of the supercoiled and nicked molecules under the influence of reactive oxygen species are estimated by agarose gel electrophoresis. The system of photochemical induction of superoxide anion by a spontaneous reoxidation of photoreduced flavin with the subsequent formation of multiple active forms of oxygen is used as a model of an oxidizing stress. The significant prevention of DNA deep degradation in the presence of alkylresorcinols, directly dependent both from length of their alkyl and from the used concentration as compared with the oxidized tests is shown. On the basis of data of infrared spectroscopy the question of possible transformations of alkylresorcinols protecting of DNAconformation in the conditions of an oxidizing stress is discussed. The experimental studies of protector effects ofalkylresorcinol from reactive oxygen species concerning to DNA molecules are forming a basis for pharmaceutical preparations development. Key words: alkylresorcinol, methylresorcinol, hexylresorcinol, dodecylresorcinol, antioxidant effect,reactive oxygen species, DNA damage, plasmid DNA pUC19.

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About this article

Authors: Korotkova A.M., Davydova O.K.

Year: 2015


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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