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April 2015, № 4 (179)



Kataeva L.V., Perunova N.B., Karpukhina N.F., Stepanova T.F., Bukharin O.V. THE BIOLOGICAL CHARACTERISTIC OF THE BACTERIA OF THE SORT AEROMONAS ALLOCATED FROM MOLLUSKS-BITINIID AND A RESERVOIRThe aim: to determine the species structure and biological properties of Aeromonas, isolated from water and mollusks family Bithyniidae. Materials and methods: mollusks and water from their habitats are the materials used in this research. The material was collected at the Iryum River (the Ob-Irtysh basin) in the summer period. 176 strains of Aeromonas bacteria were picked. Identification of bacteria was performed by matrix-assisted laser desorption/ionization — MALDI-TOF Microflex LT (Brucker Daltonics ®). The presence of hemolysin, lecithinase, plazmokoagulase and lysozyme were investigated by conventional methods. The antilysozyme activity of bacteria was determined by method of Bukharin O.V. et al. (1999), the biofilm formation — by G. O'Tool et al. (2000). The results: taxonomic composition and biological properties of bacteria of the genus Aeromonas isolated from bitiinid and their habitat (water). Mollusks allocated species such as A. veronii, A. hydrophyla, A. ichthiosmia, and out of the water — A. veronii, A. ichthiosmia, rarely — A. cavia. Isolates with hemolytic, lecithinase activity and had no lysozyme and plasma coagulation activity. Intensity of antilysozyme activity and biofilm formation of bacteria of the genus Aeromonas varied depending on the source selection cultures. Conclusion: the bacteria of the genus Aeromonas are widely represented in microsymbiocenosis of mollusks Bithyniidae and water and have a complex of biological properties, which contributes to the adaptation of microorganisms to their environment. The data obtained contribute to deciphering the mechanisms of formation, maintenance and operation of the association microparasitocenosis.Key words: associative symbiosis, microsymbiocenosis, shellfish, bacteria of the genus Aeromonas.

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About this article

Authors: Buharin O.V., Perunova N.B.

Year: 2015


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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