Kataeva L.V., Perunova N.B., Karpukhina N.F., Stepanova T.F., Bukharin O.V.
THE BIOLOGICAL CHARACTERISTIC OF THE BACTERIA OF THE SORT AEROMONAS ALLOCATED FROM MOLLUSKS-BITINIID AND A RESERVOIR ^{[№ 4 ' 2015]}
The aim: to determine the species structure and biological properties of Aeromonas, isolated from water and mollusks family Bithyniidae. Materials and methods: mollusks and water from their habitats are the materials used in this research. The material was collected at the Iryum River (the Ob-Irtysh basin) in the summer period. 176 strains of Aeromonas bacteria were picked. Identification of bacteria was performed by matrix-assisted laser desorption/ionization — MALDI-TOF Microflex LT (Brucker Daltonics ®). The presence of hemolysin, lecithinase, plazmokoagulase and lysozyme were investigated by conventional methods. The antilysozyme activity of bacteria was determined by method of Bukharin O.V. et al. (1999), the biofilm formation — by G. O'Tool et al. (2000). The results: taxonomic composition and biological properties of bacteria of the genus Aeromonas isolated from bitiinid and their habitat (water). Mollusks allocated species such as A. veronii, A. hydrophyla, A. ichthiosmia, and out of the water — A. veronii, A. ichthiosmia, rarely — A. cavia. Isolates with hemolytic, lecithinase activity and had no lysozyme and plasma coagulation activity. Intensity of antilysozyme activity and biofilm formation of bacteria of the genus Aeromonas varied depending on the source selection cultures. Conclusion: the bacteria of the genus Aeromonas are widely represented in microsymbiocenosis of mollusks Bithyniidae and water and have a complex of biological properties, which contributes to the adaptation of microorganisms to their environment. The data obtained contribute to deciphering the mechanisms of formation, maintenance and operation of the association microparasitocenosis.

Bukharin O.V., Chelpachenko O.E., Perunova N.B., Ivanova E.V., Andryuschenko S.V.
EXPERIMENTAL AND CLINICAL BASIS OF SELECTION BY ANTIMYCOTIC PHYTODRUGS ^{[№ 3 ' 2015]}
The use of antilysozyme and lipolytic activity as biotarget allowed to select herbs with maximum inhibitory effect of biological properties C.albicans: Artemisia absinthium L., Rosa cinnamomea L. infusions and Juniperus communis L., Citrus limon burm., Dianthus, Melissa officinalis Chaix. и Aloe arborescens mill aromatic substances. Conducted clinical laboratory studies have shown the efficacy of selected herbal drugs, which manifestated by reducing the frequency of intestinal dysbiosis with C.albicans hypercolonization by patients with CTD and decrease frequency somatic diseases in research group,and proved the possibility of using this method of selection antimycotic drugs.

Sirotkina I.A., Bukharina E.S.
RECONSTRUCTION FEATURES OF LOWER ORBITAL WALL BY PATIENTS WITH ANOPHTHALMIA ^{[№ 12 ' 2014]}
The features of the reconstruction of the lower wall (bottom) of the orbit in patients with anophthalmos. With the combination of severe eye injury with fracture of the bottom of the orbit performed enucleation with support locomotor stump (SLS); reconstruction of the bottom of the orbit is done the next step. In patients with anophthalmia and bottom fracture orbit the first step recovers the bottom, SLS formed the second stage. With the combination of anophthalmia and absence of the bottom of the orbit on the big stage reconstruction is performed during orbit and plastic.

Drozdova E.A., Bukharina E.S.
DIAGNOSTIC PECULIARITIES OF ISOLATED AND COMBINED CONTUSIONAL FRACTURES OF ORBITAL MEDIAL WALL ^{[№ 12 ' 2012]}
There were investigated the peculiarities of the clinic and modern diagnostic methods of isolated and combined contusional fractures of orbital medial wall. There were obtained the data supporting the need for early implementation of multispiral computed tomography — research for suspected fracture of the orbit medial wall.

Bukharin O.V., Kirgizova S.B., Kartashova O.L., Potekhina L.P.
ANTICARNOSINE ACTIVITY AND ITS ROLE AT MICROORGANISMS’ PERSISTENCE ^{[№ 12 ' 2006]}
Evidences of anticarnosine activity (ACrA) of bacteria at their persistence are got on the model of experimental staphylococcus infection. The analysis of feature’s prevalence and intensity of clinical bacteria isolate showed that staphylococcus had ACrA predominantly; at the same time the high meanings of the feature were registered at cultures, distinguished from biotope with high concentration of carnosine (olfactory epithelium of mucous tunic of nasal meatus) which had meaning at phenomenon of bacteria carrying of these microorganisms.

Bukharin O.V., Chainikova I.N., Smolyagin A.I., Valyshev A.V., Perunova N.B., Vlasova E.V., Kalinina T.N.
CHARACTERISTICS OF LOCAL BOWELS IMMUNITY AND MICROBIOCENOSE OF PATIENTS WITH SALMONELLOSIS INFECTION ^{[№ 5 (app.1) ' 2005]}
It was revealed that evident numerical and qualitative damages of microbiocenose condition of thick bowels of patients with salmonellosis are accompanied with synchronous change of local immunity characteristics in coprophiltrates and saliva.

Chainikova I.N., Smolyagin A.I., Livshits N.M., Sapozhnikova A.V., Bukharin O.V.
INFECTOLOGICAL ASPECTS OF MODEL SYSTEM OF EXPERIMENTAL SALMONELLOSIS INFECTION ^{[№ 5 (app.1) ' 2005]}
Peculiarities of infectious process of mice (CBA x C57 BL6)F1 at enteral infection with strain S. typhimurium and S. enteritidis, were studied in this article. Salmonella strain of enteritidis stimulated more expressed changes of infectious process (lethality, organs’ semination, cellular structure changes of lymphoid organs, suppression of macrophage bactericidal characteristics) and were characterized with higher level of antilactoferrin, anticomplementary, antiimmunoglobulin activity.

Yeremin M.N., Kirillov D.A., Sheenkov N.V., Bukharin O.V.
MODIFYING ACTION OF "DEZOTCELL" DRUG UPON BIOLOGICAL PROPERTIES OF MICROORGANISMS ^{[№ 1 ' 2003]}
Bactericidal action of "Dezotcell" drug upon E. coli, K. pneumoniae, S. aureus, S. enteritidis, S. typhimurium, S. flexneri, P. aeruginosa, B. abortus bovis has been ascertained experimentally in vitro. The drug influence upon antilysozyme strain activity was defined. It has been shown that the drug possesses bactericidal effect and suppresses antilysozyme activity of the investigated strains. The investigation results enable the usage of the drug as a disinfectant.