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Korneeva О.S., Bozhko О.Yu. APPLICATION OF ISOMALTULOSESYNTHASE ERWINIA RHAPONTICI FOR TRANSFORMATION OF SACCHAROSE INTO ISOMALTULOSEThe optimum conditions for biosynthesis of isomaltulosesynthase by the bacteria Erwiniа rhapontici was found in the deep cultivation: cultivation temperature 30°С; basic value рН for the nutrient medium 7,5; duration of cultivation totals 54 hrs in the medium with a saccharose content of 10%. homogeneous enzymatic drug with a s protein specific activity of 281,67 Е/mg was obtained through electrophoretic method. The optimum parameters for transformation of saccharose into isomaltulose were identified: dosage – 5 Е/mg of saccharose, saccharose concentration – 10%, transformation time – 3 hrs.
About this article
Authors: Bozhko O.Yu., Korneeva O.S.
Year: 2009
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Editor-in-chief |
Sergey Aleksandrovich
MIROSHNIKOV |
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