June 2015, № 6 (181)

Samkova E.B., Karimov I.F. ASSESMENT OF LIGHTING SOURCES BIOSAFETY BY REPORTER LUMINESCENT BACTERIAAbstract. In recent years a number of studies purpose was to develop a biosensor methods for the evaluation of mutagenic and carcinogenic effects of environmental factors on the cell, in particular biosensor test systems whole cells that have in their composition two main genetic elements: a promoter that responds to exposure to a toxicant, and gene-reporter transcribed from the promoter. It may be noted that reporter microorganisms very suitable object for the study of DNA-damaging effects, due to ease of cultivation, inexhaustible resources, to ease of changing the irradiation conditions, and finally the ability to transcriptional response that is easy to visualize the level of luminescence of the bacterial strain. In this work reporter luminescent strains that are based on E. coli K12 MG1655: E. coli K12 MG1655 recA::lux, E. coli K12 MG1655 colD::lux and E. coli K12 MG1655 ibpA::lux were used to assess the biosafety sources artificial light, with the first two luminescence respond to DNA damage, and the latter — on the damage of heat shock proteins. For creating optimal conditions for the work of strains was established method of estimating the light, and a comparison of the physical characteristics of light sources, including incandescent lamps, compact fluorescent lamps and LED lamps. The results allowed to state that all types of lamps are able to provide the DNA damaging effects in different degrees, but more important is the brand, rather than the type of lamp; wherein a correlation damage from ultraviolet radiation intensity emitted by all types of light sources. There was also a difference in the response of the features of the strains E. coli recA::lux and E. coli colD::lux.Key words: reporter strains, compact fluorescent lamp, incandescent, LED lamp, ultraviolet, DNA damage.

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References:

1 Aizenberg, Iu. B. Sovremennye problemy energoeffektivnogo osveshcheniia / Iu.B. Aizenberg // Energosberezhenie. — 2009. — № 1. — P. 42–47.

2 Gribkov, O.I. Issledovanie istochnikov sveta : Metodicheskie ukazaniya k laboratornoj rabote № 32 / O.I. Gribkov, A.V. Volkov, O.A. Ustinov. — Moskva: MIIT, 2006. — P. 48.

3 Rossiiskaia Federatciia. Zakony. Ob energosberezhenii, o povyshenii energeticheskoi effektivnosti i o vnesenii izmenenii v otdelnye zakonodatelnye akty Rossiiskoi Federatcii : feder. zakon RF № 261-FZ : [priniat Gos. Dumoi 11 november 2009: odobren Sovetom Federatcii 18 november 2009].

4 Abramova, L.V. Psixofiziologicheskaya i gigienicheskaya ocenka osveshheniya vy'sokoe'ffektivny'mi istochnikami sveta / L.V. Abramova // Svetotexnika. — 2001. — № 3. — P. 11–15.

5 Mironava, T. The effects of UV emission from compact fluorescent light exposure on human dermal fibroblasts and keratinocytes in vitro / T. Mironava, M. Hadjiargyrou, M. Simon, M.H. Rafailovich // Photochemistry and photobiology. — 2012. — V. 88. — № 6. — P. 1497–1506.

6 Light sensitivity [Электронный ресурс]. / Scientific committee on emerging and newly identified health risks. — 2008. — Режим доступа : http://ec.europa.eu/health/ph_risk/risk_en.htm. — 23.09.2008.

7 Health effects of artificial light [Электронный ресурс]. / Scientific committee on emerging and newly identified health risks. — 2012. — Режим доступа : http://ec.europa.eu/health/scientific_committees/policy/index_en.htm. — 19.03.2012.

8 Van der Lelie, D. The use of biosensors for environmental monitoring / D. Van der Lelie, P. Corbisier, E. Thiry, L. Diels // Res. Microbiol. 1994. — V. 145. — № 1. — P. 67–74.

9 Robbens, J. Escherichia coli as a bioreporter in ecotoxicology / J. Robbens, F. Dardenne, L. Devriese, W. De Coen, R. Blust // Appl. Microbiol. Biotechnol. — 2010. — V. 88. — № 5. — P. 1007-1025.

10 Ushakov, V. Yu. SOS-sistema reparacii DNK u bakterij / V.Yu. Ushakov // Vestnik Permskogo Universiteta. — 2010. — № 2. — P.19-25

11 Kisker, C. Prokaryotic nucleotide excision repair / C. Kisker, J. Kuper, B. Van Houten // Cold spring harbor perspectives in biology. — 2013. — V. 5. — № 3. — P. 1259-1263.

12 Lu, D. Structural basis of Escherichia coli single-stranded DNA-binding protein stimulation of exonuclease I / D. Lu, J.L. Kec // Proceedings of the National Academy of Sciences. — 2008. — V. 105. — № 27. — P. 9169–9174.

13 Norman, A. Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, um

UDC, or sulA promoters / A. Norman, L.H. Hansen, S.J. Sorensen // Applied and environmental microbiology. — 2005. — V. 71. — № 5. — P. 2338–2346.

14 Kotova, V. Yu., Lux-biosensors for detection of SOS-response, heat shock, and oxidative stress / V. Yu. Kotova, I.V. Manukhov, G. B. Zavil'gel'skii // Applied biochemistry and microbiology. — 2010. — T. 46. — №. 8. — P. 781–788.

15 Manukhov, I.V. Induction of oxidative stress and SOS response in Escherichia coli by plant extracts: the role of hydroperoxides and the synergistic effect of simultaneous treatment with cisplatinum / I.V. Manukhov, V. Yu. Kotova, D. G. Mal'dov, A. V. Il'ichev, A. P. Bel'kov, G. B. Zavil'gel'skii // Mikrobiologiia. — 2007. — V. 77. — № 5. — P. 590-597.

About this article

Authors: Samkova E.B., Karimov I.F.

Year: 2015

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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