december 23, 2024

RU/EN

Headings of Vestnik

October 2017, № 9 (209), pages 70–75

doi: 10.25198/1814-6457-209-70

Kochkina E.E., Pashkova T.M., Sycheva M.V., Kartashova O.L. CHARACTERISTICOF BACTERIA OF THE GENUS ENTEROCOCCUS BIOPROFILES ISOLATED FROM ANIMALSThe increasing clinical significance of enterococci highlights the issue of the safety of their use as anti-infective biologics probiotic and requires the search algorithm for the effective differentiation of clinically significant strains from representatives metallisticheskoy microbiota.
Studied the biological properties of bacteria of the genus Enterococcus isolated from the feces of clinically healthy productive animals and from animals with infectious and inflammatory diseases. Determined bioprofile characterizing pathogenic and non-pathogenic isolates. In addition to the genetic determinants of the virulence factors of the strains isolated in the pathology detected hemolytic, gelatinase activity and the factors of persistence (ALA, Acre), with higher characteristic values than fecal cultures. Strains isolated from the intestines of healthy animals was characterized by a high activity of proteases and not had the ability to form biofilm.
The obtained results can be used for differentiation of pathogenic variants Enterococcus spp. from symbiotic cultures included in the composition of the intestinal normality.Key words: Enterococcus spp., differentiation, factors of persistence, factors of virulence, polymerase chain reaction.

Download

References:

1. Ashmarin, I.P. Statistic methods in microbiology / I.P.Ashmarin, A.A.Vorobyov. — L.: Gos.isd. med. lit. — 1962.–177 с.

2. Bondarenko, V.М. Symbiotic enterococci and enterococcal problems opportunistic infections / V.М. Bondarenko, А.N. Suvorov. — М.:Medicine, 2007. — 30 p.

3. Bukharin, O.V. The method of determining anticorrosive activity of microorganisms / О.V. Bukharin, О.L. Chernova, S.B. Matyushina // Patent № 2132879. — 1999. — No. 19.

4. Bukharin, O.V. Photometric determination of antilysozyme activity of microorganisms / O.V. Bukharin, А.V. Valyshev, N.N. Elagina // JournalofMicrobiology, epidemiologyandImmunobiology. — 1997. — No. 4. — P. 117-120.

5. Valysheva, I.V. Genetic characterization of the virulence potential of enterococci of the intestinal microbiota of man / I.V. Valysheva // Journal of Microbiology, epidemiology and Immunobiology. — 2012. — No. 4. — P. 44-47.

6. Netrusov, A.I. Workshop on Microbiology / A.I.Netrusov. — М.:Academia, 2005. — 608 p.

7. Poshvina, D.V.Species characteristics and proteolytic activity of enterococci / D.V. Poshvina, M.V. Sycheva// Journal of veterinary medicine. — 2014. — No. 2(69). — P. 40-43.

8. Guide to microbiological and virological research methods / ed. M.O. Birger. — M.: Medicine, 1982. — 464 p.

9. Sycheva, M.V. Method of differentiation of enterococci of the intestinal microflora of animals / M.V. Sycheva, N.E.Shchepitova,O.L. Kartashova[et al.] // Patent № 2612141. — 2017. –№ 7.

10. Shchepitova, N.E.Biological properties antagonistically active enterococcimicroflora of the intestine of animals / N.E.Shchepitova, M.V.Sycheva, O.L. Kartashova// Vestnik of Orenburg state University. — 2014. — No. 13. — P. 134-138.

11. AntimicrobialresistanceandvirulencetraitsinEnterococcusstrainsisolatedfromdogsandcats / R. Iseppi, P. Messi, I. Anacarso [et al.] // New Microbiol. — 2015. — Vol. 38(3). –P. 369-378.

12. Coburn, P.S. The Enterococcus faecalis cytolysin: a novel toxin active against eukaryotic and prokaryotic cells / P.S. Coburn, M.S. Gilmore // Cell Microbiol. — 2003. — Vol. 5 — No.10. — P. 661-669.

13. Development of Multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in Enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium / V. Vankerckhoven, T. Van Autgaerden, C. Vael [et al.] // Journal of Clinical Microbiology. — 2004. — No. 42. — P. 4473-4479.

14. Effects of Enterococcus faecalis for genes on production of gelatinase and a serine protease and virulence / X. Qin, K.V. Singh, G.M. Weinstock [et al.] // Infect. Immun. — 2000. — Vol. 68. — P. 2579-2586.

15. Enterococcus faecalis clones in poultry and in humans with urinary tract infections, Vietnam / L.L. Poulsen, M. Bisgaard, N. Son [et al.] // Emerging Infectious Diseases. — 2012. — Vol. 18. — No. 7. — P. 1096-1100.

16. Gaggı`a, F. Probiotics and prebiotics in animal feeding for safe food production / F. Gaggı`a, P. Mattarelli, B. Biavati // Int. J. Food Microbiol. — 2010. — Vol. 141. — P. 15-28.

17. Kafil, H.S. Spread of Enterococcal Surface Protein in Antibiotic Resistant Enterococcus faecium and Enterococcus faecalis isolates from Urinary Tract Infections / H.S. Kafil, A.M. Mobarez // Open Microbiol. J. — 2015. — Vol. 26. — No. 9. — P. 14-17.

18. Keis, S. Characterization of a vancomycinresistant Enterococcus faecalis (VREF) isolate from a dog with mastitis: further evidence of a clonal lineage of VREF in New Zealand / S. Keis, J.M. Smith, G.M. Cook // J. Clin. Microbiol. — 2003. — Vol. 13. — P. 3331-3333.

19. O’Toole, G.A. Biofilm formation as microbial development / G.A. O’Toole, H.B. Kaplan, R. Kolter // Annual Review of Microbiology. — 2000. — No. 54. — P. 49-79.

20. Phenotypic and genotypic study of gelatinase and beta-haemolysis activities in faecal enterococci of poultry in Portugal / P. Poeta, D. Costa, N. Klibi [et al.] // J. Vet. Med. — 2006. — Vol. 53. — No. 5. — P. 203-208.

21. Pomba, C. Treatment of a lower urinary tract infection in a cat caused by a multi-drug methicillin-resistant Staphylococcus pseudintermedius and Enterococcus faecalis / C. Pomba, N. Couto, A.J. Moodley // Feline Med. Surg. — 2010. — Vol. 12 — No. 10. — P. 802-806.

22. Screening of virulence determinants in Enterococcus faecium strains isolated from breast milk / C. Reviriego, T. Eaton, R. Martín [et al.] // Journal of Human Lactation. — 2005. — No. 21. — P. 131-137.

23. Virulence factors in food, clinical and reference enterococci: a common trait in the genus? / T. Semedo, M.A. Santos, M.F. Lopes [et al.] // Systematic and Applied Microbiology. — 2003. — No. 26. — P. 13-22.

24. Virulence traits and antibiotic resistance among enterococci isolated from Eurasian otter (Lutralutra) / T. Semedo-Lemsaddek, C.S. Nóbrega, T. Ribeiro [et al.] // Vet. Microbiol. — 2013. — Vol. 163. — No. 3-4. — P. 378-382.

25. Yuen, G.J. Enterococcus infection biology: Lessons from invertebrate host models / G.J. Yuen, F.M. Ausubel // J. Microbiol. — 2014. — Vol. 52. — No. 3. — P. 200-210.

About this article

Authors: Kochkina E.E., Pashkova T.M., Sycheva M.V., Kartashova O.L.

Year: 2017

doi: 10.25198/1814-6457-209-70

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

© Электронное периодическое издание: ВЕСТНИК ОГУ on-line (VESTNIK OSU on-line), ISSN on-line 1814-6465
Зарегистрировано в Федеральной службе по надзору в сфере связи, информационных технологий и массовых коммуникаций
Свидетельство о регистрации СМИ: Эл № ФС77-37678 от 29 сентября 2009 г.
Учредитель: Оренбургский государственный университет (ОГУ)
Главный редактор: С.А. Мирошников
Адрес редакции: 460018, г. Оренбург, проспект Победы, д. 13, к. 2335
Тел./факс: (3532)37-27-78 E-mail: vestnik@mail.osu.ru