Kartashova O.L., Pashkova T.M., Pashinina O.A.
INFLUENCE OF MEDICINAL PREPARATIONS OF PLANT ORIGIN ON ANTILIZOZYMIC ACTIVITY OF PRIORITY TYPES OF UROFLORA ALLOCATED FROM UROLITHIASIS PATIENTS ^{[№ 9 ' 2017]}
One of the most common urological diseases is urolithiasis, the therapy of which uses herbal medicines, effective in the presence of a disease associated with the inflammation in the urinary tract. A pathogenetically determined relationship between the presence of urinary stones and the occurrence of a chronic urinary infection is established in which the causative agent has a set of persistent properties that allow it to persist for a long time in the host organism. Phytopreparations have a different effect on the body, which is due to the biologically active substances that make up their composition. At the same time, the effect of herbal medicines on the persistent potential of microorganisms, in particular, antilysozyme activity, which is one of its significant factors, has not been studied.
In this connection, the effect of a number of phytopreparations, differing in composition and used for the treatment and prevention of urinary tract infections, on the antilysozyme activity of priority uroflora species isolated from patients with urolithiasis (P. aeruginosa and E. coli) was studied in vitro. The study of the possibility of phytopreparation regulation of the antilysozyme sign of bacteria in an in vitro experiment revealed their unidirectional inhibitory effect and found that the maximum reduction in antilysozyme activity in P. aeruginosa occurred under the action of the drug Gentos, and in E. coli — Kanefron.
Thus, the revealed ability of phytopreparations to unilaterally reduce the antilysozyme activity of Pseudomonas sp. and Escherichia sp. under in vitro conditions can be considered as one of the possible mechanisms of their biological activity in vivo.

Kochkina E.E., Pashkova T.M., Sycheva M.V., Kartashova O.L.
CHARACTERISTICOF BACTERIA OF THE GENUS ENTEROCOCCUS BIOPROFILES ISOLATED FROM ANIMALS ^{[№ 9 ' 2017]}
The increasing clinical significance of enterococci highlights the issue of the safety of their use as anti-infective biologics probiotic and requires the search algorithm for the effective differentiation of clinically significant strains from representatives metallisticheskoy microbiota.
Studied the biological properties of bacteria of the genus Enterococcus isolated from the feces of clinically healthy productive animals and from animals with infectious and inflammatory diseases. Determined bioprofile characterizing pathogenic and non-pathogenic isolates. In addition to the genetic determinants of the virulence factors of the strains isolated in the pathology detected hemolytic, gelatinase activity and the factors of persistence (ALA, Acre), with higher characteristic values than fecal cultures. Strains isolated from the intestines of healthy animals was characterized by a high activity of proteases and not had the ability to form biofilm.
The obtained results can be used for differentiation of pathogenic variants Enterococcus spp. from symbiotic cultures included in the composition of the intestinal normality.

Pashkova T.M.
CHARACTERISTICS OF ANTI-CYTOKINE ACTIVITY OF ENTEROCOCCUS SPP., ISOLATED FROM ANIMALS ^{[№ 9 ' 2017]}
Enterococcus spp. are typical synanthropic microorganisms that, during evolution, have adapted well to survival in a macroorganism, including through the ability to inactivate the factors of innate immunity. Possessing the factors of persistence, the bacteria of the genus Enterococcus can act as the etiological factor of endogenous infectious and inflammatory diseases of animals that arise against the background of a decrease in the natural resistance of the macroorganism. A number of studies have been devoted to the characterization of the persistent properties of Enterococcus spp. of various origin, but certain properties of Enterococcus spp., in particular, anticytokine activity, have not yet been characterized.
In this regard, the anti-cytokine activity of microorganisms of the genus Enterococcus isolated from healthy and infectious-inflammatory diseases (acute and chronic endometritis, mastitis, soft tissue abscesses) in agricultural animals has been studied and the high prevalence of the trait is shown. A higher level of anti-cytokine activity against IL-4 and IL-8 was detected in Enterococcus spp. isolates isolated from infectious inflammatory diseases in comparison with strains isolated from the intestines of healthy animals. Expression of anti-cytokine activity in clinical enterococci was 1.5–1.7 times higher than in intestinal isolates (p < 0.05). Data on the high level of anti-cytokine activity in clinical isolates Enterococcus spp. in comparison with intestinal ones testify to the possible influence of their exometabolites on the local cytokine balance, which supports the inflammatory process.
Thus, the results obtained on the modification of Enterococcus spp. of different origin of cytokine dynamics can be used to differentiate pathogenic strains of Enterococcus spp.