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November 2017, № 12 (212), pages 84–89

doi: 10.25198/1814-6457-212-84

Vasilchenko A.S., Valyshev A.V. OPTIMIZATION OF A CULTURE MEDIUM COMPOSITION AS APROACH TO ISOLATION OF BACTERIOCINSAntimicrobial peptides of bacterial origin can be considered as effective food preservatives, as well as therapeutic agents. The first difficulties in the study of these substances begin at the stage of bacteriocin`s isolation from the culture medium of the producer microorganism. A simple and affordable approach for optimizing isolation of bacteriocins from metabolites of microorganisms is presented. In this study a strain of E. faecium ICIS 7 was used as bacteriocin producer. Bacteria were cultured on four different media comprising two commercial medium and two semisynthetic. Isolation of bacteriocins was carried out by reversed-phase liquid high-performance chromatography. At the first stage, the bacterial growth parameters on various media were estimated and bacteriocin`s production was evaluated semi-quantitatively. At the second stage chromatographic profiles were obtained and compared. Finally it was shown that using a simple media consisting of the yeast extract only, makes it possible to produce bacteriocins and subsequently simplify their chromatographic purification. Thus, it can be concluded that the yeast extract is an ingredient available to most laboratories, while the components for the preparation of the chemical-defined medium are still quite expensive chemical reagents (amino acids, nucleic acids etc.). The use of a yeast extract for the cultivation and production of bacteriocins significantly reduces the cost of isolating the target polypeptide.Key words: bacteriocin, simple medium, chemically defined medium.

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References:

1. Ventola C.L. The Antibiotic Resistance Crisis: Part 1: Causes and Threats // Pharmacy and Therapeutics. — 2015, — V.40 — N 4, pp. 277-283.

2. Brogden K. Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? // Nat Rev Microbiol. — 2005, –V.3, — pp. 238–50.

3. Cytryńska M., Zdybicka-Barabas A. Defense peptides: recent developments // Biomol Concepts, — V. 6, N.4 pp. 237-51.

4. Wang G., Li X., Wang Z. APD3: the antimicrobial peptide database as a tool for research and education // Nucleic Acids Research, — 2016, — V.44, — pp. D1087-D1093.

5. Korneva E.A., Kokryakov V.N. Defensins: Antimicrobial peptides with a broad spectrum of biological activity // NeuroImmune Biology — 2007, — V. 3, — pp.451–462.

6. Cheigh C.I., Kook M.C., Kim S.B., Hong Y.H., Pyun Y.R. Simple one-step purification of nisin Z from uncalrified culture broth of Lactococcus lactis subsp. lactis A164 using expanded bed ion exchange chromatography // Biotechnol Lett — 2004 — V.26, pp. 1341–1345.

7. Li C, Bai J, Cai Z, Ouyang F. (2002) Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology. J Biotechnol: 93:27–34.

8. Garsa A.K., Kumariya R., Sood S.K. et al. Bacteriocin Production and Different Strategies for Their Recovery and Purification // Probiotics & Antimicro Prot: — 2014, — V.6, — p. 47.

9. Fimland G., Sletten K., Nissen-Meyer J. The complete amino acid sequence of the pediocin-like antimicrobial peptide leucocin C // Biochem Biophys Res Commun, — 2002, — V. 295, — pp. 826–827.

10. Chumchalova J., Stiles J., Josephsen J., Plockova M. Characterization and purification of acidocin CH5, a bacteriocin produced by Lactobacillus acidophilus CH5. // J Appl Microbiol, — 2004, — V. 96, — pp.1082–1089.

11. Alfoldi L. La production induite de megacine en milieu synthetique // Ann Inst Pasteur, — 1957, — V. 94 — P. 474.

12. Pingitore V.E., Salvucci E., Sesma F., Nader-Macías M.E. Different strategies for purification of antimicrobial peptides from Lactic Acid Bacteria (LAB). Communicating Current Research and Educational Topics and Trends in Applied Microbiology A. Méndez-Vilas(Ed). 2007.

13. Medaglia G., Panke S. Development of a fermentation process based on a defined medium for the production of pregallidermin, a nontoxic precursor of the lantibiotic gallidermin // Appl Microbiol Biotechnol, — 2010, — V.87, — pp.145–157.

14. Pingitore E.V., Hebert E.M., Sesma F., Nader-Macias M.E. Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium // Can J Microbiol, — 2009, –V. 55, — pp. 304–310.

15. Moretro T., Hagen B.F., Axelsson L. A new, completely defined medium for meat lactobacilli // J Appl Microbiol, — 2008, — V. 85, — pp.715–722.

16. Byappanahalli M.N., Nevers M.B., Korajkic A., Staley Z.R., Harwood V.J. Enterococci in the environment //Microbiol Mol Biol Rev, — 2012, — V. 76, — N4, — pp.685–706.

17. Yamamoto Y., Togawa Y., Shimosaka M., Okazaki M. Purification and Characterization of a Novel Bacteriocin Produced by Enterococcus faecalis Strain RJ-11 // Appl Environ Microbiol, — 2003, — V. 69, — pp. 5746-5753.

18. Vasilchenko A.S., Rogozhin E.A., Valyshev A.V. Purification of a novel bacteriocin-like inhibitory substance produced by Enterococcus faecium ICIS 8 and characterization of its mode of action // Microbial Drug Resistance, — 2016, — pp. 1-10, DOI: 10.1089/mdr.2016.0069.

19. Kuipers O.P., Beerthuyzenm M.M., de Ruyter P.G.G.A., Luesink E.J., de Vos W.M. Autoregulation of nisin biosynthesis in Lactococcus lactis by signal transduction // J Biol Chem, — 1995, — V.270, — pp.27299–304.

20. Ivanova I., Miteva V., Stefanova T., Pantev A., Budakov I., Danova S., Moncheva P., Nikolova I., Dousset X., Boyaval P. Characterization of a bacteriocin produced by Streptococcus thermophilus 81 // Int J Food Microbiol, — 1998, — V. 42, — pp.147–158.

21. Khan H., Flint S.H., Yu P.-L. Development of a chemically defined medium for the production of enterolysin A from Enterococcus faecalis B9510 // J Appl Microbiol, — 2013, V. 114, — pp.1092–1102.


About this article

Authors: Vasilchenko A.S., Valyshev A.V.

Year: 2017

doi: 10.25198/1814-6457-212-84

Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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