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October 2017, № 9 (209), pages 85–90

doi: 10.25198/1814-6457-209-85

Polyudova T.V., Yurkina N.O., Eroshenko D.V., Korobov V.P. INFLUENCE OF BLOOD COMPONENTS ON ADHESION OF NON-TUBERCULOUS MICROBACTERIACurrently, diseases caused by non-tuberculosis mycobacteria (NTMB) are increasingly diagnosed in people with reduced immune competence. The most common form of diseases associated with NTMB is infection caused by the biofilm formation on the surface of long-term medical devices. However, the mechanisms of adhesion of NTMB on abiotic surfaces upon contact with internal media of the organism are still unclear.
The effect of human plasma and hemoglobin, fetal bovine serum (FBS), bovine serum albumin (BSA), fibronectin, DNA, lysozyme and lactoferrin on the adhesion of Mycobacterium smegmatis mc² 155, M. smegmatis GISK 607, M. avium GISK 168 to polystyrene has been studied. The presence of plasma (10 %), serum (10 %), albumin (0.5 mg/ml), lysozyme (0.1 mg/ml), and lactoferrin (0.1 mg/ml) significantly reduced the amount of adherent cells of all strains studied. The greatest suppressing effect on mycobacteria was detected in the presence of BSA and FBS. The presence of cattle DNA (0.5 mg/ml) into the incubation medium also resulted in a significant decrease in the number of adherent cells. However, the introduction of fibronectin (2.5 mg/ml) and hemoglobin into the medium revealed heterogeneity among NTMBs for the ability to bind to the polystyrene. Thus, the adhesion of M. smegmatis GISK 607 and M. avium HISK 168 was enhanced in the presence of fibronectin and did not change for M. smegmatis mc² 155. M. Hemoglobin has both inhibitory (M. smegmatis mc² 155 and M. avium GISK 168) and stimulating (M. smegmatis GISK 607) effect on the adhesion of NTMB.
The obtained data about the influence of blood components on the attachment of NTMB to the abiotic surface allow us to indirectly judge the biofilm formation in vivo.Key words: adhesion, albumin, DNA, non-tuberculous microbacteria, serum blood.

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References:

1. Rapidly growing mycobacterial bloodstream infections / G. El Helou, G.M. Viola, R.Hachem [et al] // Lancet Infect. Dis. — 2013. — V. 13. — P.166-174.

2. Litvinov, V.I. Non-tuberculous mycobacteria in “Non-living and living nature”, human infection / V.I. Litvinov // Tuberculosis and socially significant diseases. — 2015. — No. 2. — P. 28-33.

3. Relationships between Mycobacterium isolates from patients with pulmonary mycobacterial infection and potting soils / M.A. De Groote, N.R. Pace, K. Fulton [et al] // Appl. Environ. Microbiol. — 2006. — No. 72. — Р. 7602-7606.

4. Mullis, S.N. Adherence and biofilm formation of Mycobacterium avium, Mycobacterium intracellulare and Mycobacterium abscessus to household plumbing materials / S.N. Mullis, J.O. Falkinham // J. Appl. Microbiol. — 2013. — V. 115. — No. 3. — P. 908-914.

5. Structural analysis of biofilm formation by rapidly and slowly growing nontuberculous mycobacteria / M.M. Williams, M.A. Yakrus, M.J. Arduino [et al] // Appl. Environ. Microbiol. — 2009. — V. 75. — No. 7. — P. 2091-2098.

6. Mycobacterium fortuitum prosthetic valve endocarditis: a case for the pathogenetic role of biofilms / S. Bosio, S. Leekha, S.I. Gamb [et al] // Cardiovasc. Pathol. — 2011. — V. 21. — P. 361-364.

7. Venous catheter-associated bacteremia caused by rapidly growing mycobacteria at a medical center in central Taiwan / C.Y. Chang, R.W. Tsay, L.C. Lin [et al] // J. Microbiol. Immunol. Infect. — 2009. — V. 42. — P. 343-350.

8. Fibronectin, fibrinogen, and laminin act as mediators of adherence of clinical staphylococcal isolates to foreign material / M. Herrmann, P.E. Vaudaux, D. Pittet [et al] // J. Infect. Dis. — 1988. — V. 158. — No. 4. — P. 693–701.

9. Modes of conformational changes of proteins adsorbed on a planar hydrophobic polymer surface reflecting their adsorption behaviors / R. Ishiguro, Y. Yokoyama, H. Maeda [et al] // J. Colloid Interface Sci. — 2005. — V. 290. — P. 91-101.

10. Turna, A.A. Problems of determination of hemoglobin in laboratory diagnostics and measures for its solution / A.A. Turna // Laboratory diagnostics. Special edition “Laboratory”. — 2013. — No. 3. — P. 48-53.

11. Hymes, J.P. Stuck in the Middle: Fibronectin-Binding Proteins in Gram-Positive Bacteria/ J.P. Hymes, T.R. Klaenhammer // Front. Microbiol. — 2016. — V. 7. — Р. 1504-1513.

12. Fibronectin-binding proteins secreted by Mycobacterium avium / H. Kitaura, N. Ohara, M. Naito [et al] // APMIS. — 2000. — V.107. — No. 9. — P.558-564.

13. Eroshenko, D. The Role of Plasma, Albumin, and Fibronectin in Staphylococcus epidermidis Adhesion to Polystyrene Surface / D. Eroshenko, I. Morozov, V. Korobov // Curr. Microbiol. — 2015. — V. 70. — P.846–853.

14. Hemoglobin promotes Staphylococcus aureus nasal colonization / M. Pynnonen, R.E. Stephenson, K. Schwartz [et al] // PLoS Pathog. — 2011. — V. 7. — No. 7. — Р. 1-11.

15. Alpha and beta chains of hemoglobin inhibit production of Staphylococcus aureus exotoxins / P.M. Schlievert, L.C. Case, K.A. Nemeth [et al] // Biochemistry. — 2007. — V. 46. — No. 50. — Р.14349-58.

16. Antibacterial hemoglobin peptides in human menstrual blood / P. Mak, K. Wójcik, L. Wicherek [et al] // Peptides. — 2004. — V. 25. — No. 11. — Р. 1839-1847.

17. Bacterial adherence to titanium surface coated with human serum albumin / T.J. Kinnari, L.I. Peltonen, P. Kuusela [et al] // Otol. Neurotol. — 2005. — V. 26. — No. 3. — P. 380-384

18. Quantitation and characterization of competitive protein binding to polymers / C.N. Cottonaro, H.V. Roohk, G. Shimizu [et al] // Trans. Am. Soc. Artif. Intern. Organs. — 1981. — V. 27. — P. 391-395.

19. Gahan, P.B. Circulating nucleic acids in plasma and serum. Recent developments / P.B. Gahan, R. Swaminathan // Ann. N.Y. Acad. Sci. — 2008. — V. 1137. — P.1-6.

20. Extracellular DNA in adhesion and biofilm formation of four environmental isolates: a quantitative study / L. Tang, A. Schramm, T.R. Neu [et al] // FEMS Microbiol Ecol. — 2013. — V. 86. — No. 3. — P. 394-403.

21. Gilan, I. Extracellular DNA Plays an Important Structural Role in the Biofilm of the Plastic Degrading Actinomycete Rhodococcus ruber / I. Gilan, A. Sivan // Adv. Microbiol. — 2013. — V. 3. — P. 543–551

22. Eroshenko, D.V. Influence of environmental factors on the first stages of biofilm formation by Staphylococcus epidermidis / D.V. Eroshenko // PhD thesis. IEGM UB RAS, Perm, 2015. — 137 p.

23. Inhibition of initial bacterial adhesion on titanium surfaces by lactoferrin coating / F. Nagano-Takebe, H. Miyakawa, F. Nakazawa [et al] // Biointerphases. — 2014. — V. 9. — No. 2. — P. 029006.

24. Influence of protein deposition on bacterial adhesion to contact lenses / L.N. Subbaraman, R. Borazjani, H. Zhu [et al] // Optom. Vis. Sci. — 2011. — V.88. — No. 8. — P. 959-966.

About this article

Authors: Polyudova T.V., Yurkina N.O., Eroshenko D.V., Korobov V.P.

Year: 2017

doi: 10.25198/1814-6457-209-85

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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