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October 2017, № 9 (209), pages 85–90

doi: 10.25198/1814-6457-209-85

Polyudova T.V., Yurkina N.O., Eroshenko D.V., Korobov V.P. INFLUENCE OF BLOOD COMPONENTS ON ADHESION OF NON-TUBERCULOUS MICROBACTERIACurrently, diseases caused by non-tuberculosis mycobacteria (NTMB) are increasingly diagnosed in people with reduced immune competence. The most common form of diseases associated with NTMB is infection caused by the biofilm formation on the surface of long-term medical devices. However, the mechanisms of adhesion of NTMB on abiotic surfaces upon contact with internal media of the organism are still unclear.
The effect of human plasma and hemoglobin, fetal bovine serum (FBS), bovine serum albumin (BSA), fibronectin, DNA, lysozyme and lactoferrin on the adhesion of Mycobacterium smegmatis mc² 155, M. smegmatis GISK 607, M. avium GISK 168 to polystyrene has been studied. The presence of plasma (10 %), serum (10 %), albumin (0.5 mg/ml), lysozyme (0.1 mg/ml), and lactoferrin (0.1 mg/ml) significantly reduced the amount of adherent cells of all strains studied. The greatest suppressing effect on mycobacteria was detected in the presence of BSA and FBS. The presence of cattle DNA (0.5 mg/ml) into the incubation medium also resulted in a significant decrease in the number of adherent cells. However, the introduction of fibronectin (2.5 mg/ml) and hemoglobin into the medium revealed heterogeneity among NTMBs for the ability to bind to the polystyrene. Thus, the adhesion of M. smegmatis GISK 607 and M. avium HISK 168 was enhanced in the presence of fibronectin and did not change for M. smegmatis mc² 155. M. Hemoglobin has both inhibitory (M. smegmatis mc² 155 and M. avium GISK 168) and stimulating (M. smegmatis GISK 607) effect on the adhesion of NTMB.
The obtained data about the influence of blood components on the attachment of NTMB to the abiotic surface allow us to indirectly judge the biofilm formation in vivo.Key words: adhesion, albumin, DNA, non-tuberculous microbacteria, serum blood.

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About this article

Authors: Polyudova T.V., Yurkina N.O., Eroshenko D.V., Korobov V.P.

Year: 2017

doi: 10.25198/1814-6457-209-85

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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