Klimova T.A., Barysheva E.S., Morozova N.V., Korobova I.V.
APPLICATION OF PROBIOTIC PREPARATIONS BASED ON BACILLUS OF THE GENUS BACILLUS IN THE IRON AND ZINC SYSTEM OF DELIVERY IN THE ORGANISM OF LABORATORY ANIMALS ^{[№ 9 ' 2017]}
Studies in the field of iron and zinc deficiency are caused by the high prevalence of iron deficiency and deficient zinc states according to official statistics of the Ministry of Health of the Russian Federation. It is known that metals in comparison with other elements have the ability to bioaccumulate. Microorganisms are able to extract and concentrate metals, in particular, this ability is possessed by microorganisms that are part of probiotic preparations based on bacteria of the genus Bacillus.
The results of the study of the possibility of delivering iron and zinc to the organism of laboratory animals using probiotic preparations based on bacteria of the genus Bacillus are presented. The probiotic strains were the living and inactivated bacteria of the genus Bacillus: B. subtilis 534, B. cereus 5832, B. licheniformis 7038 and B. subtilis 7048. The data obtained indicate that in the iron delivery system, the most effective probiotic strains studied are inactivated with B. licheniformis 7038 and B. subtilis 7048 (increase in iron in the skin of laboratory animals by 31.7 %, in bone tissue — 80.8 %, in muscle tissue — 75.9 %). In the zinc delivery system, the B. subtilis strain 534 proved to be the most effective, while the zinc content in the skin of laboratory animals increased by 60 %, in bone tissue by 78.5 %, and in muscle tissue by 43.6 %.
In the iron delivery system, the most effective of the drugs being tested is “Vetom-2”, and the least “Sporobacterin”. In the zinc delivery system, the most effective is “Sporobacterin”, and the least effective is “Bactisubtil”.

Polyudova T.V., Yurkina N.O., Eroshenko D.V., Korobov V.P.
INFLUENCE OF BLOOD COMPONENTS ON ADHESION OF NON-TUBERCULOUS MICROBACTERIA ^{[№ 9 ' 2017]}
Currently, diseases caused by non-tuberculosis mycobacteria (NTMB) are increasingly diagnosed in people with reduced immune competence. The most common form of diseases associated with NTMB is infection caused by the biofilm formation on the surface of long-term medical devices. However, the mechanisms of adhesion of NTMB on abiotic surfaces upon contact with internal media of the organism are still unclear.
The effect of human plasma and hemoglobin, fetal bovine serum (FBS), bovine serum albumin (BSA), fibronectin, DNA, lysozyme and lactoferrin on the adhesion of Mycobacterium smegmatis mc² 155, M. smegmatis GISK 607, M. avium GISK 168 to polystyrene has been studied. The presence of plasma (10 %), serum (10 %), albumin (0.5 mg/ml), lysozyme (0.1 mg/ml), and lactoferrin (0.1 mg/ml) significantly reduced the amount of adherent cells of all strains studied. The greatest suppressing effect on mycobacteria was detected in the presence of BSA and FBS. The presence of cattle DNA (0.5 mg/ml) into the incubation medium also resulted in a significant decrease in the number of adherent cells. However, the introduction of fibronectin (2.5 mg/ml) and hemoglobin into the medium revealed heterogeneity among NTMBs for the ability to bind to the polystyrene. Thus, the adhesion of M. smegmatis GISK 607 and M. avium HISK 168 was enhanced in the presence of fibronectin and did not change for M. smegmatis mc² 155. M. Hemoglobin has both inhibitory (M. smegmatis mc² 155 and M. avium GISK 168) and stimulating (M. smegmatis GISK 607) effect on the adhesion of NTMB.
The obtained data about the influence of blood components on the attachment of NTMB to the abiotic surface allow us to indirectly judge the biofilm formation in vivo.

Tsibina А.S., Lozovaya G.F., Sankov A.N., Dydarenkova M.R., Korobov A.M.
OPTIMIZATION OF MEDICAL ASSISTANCE TO CHILDREN WITH RESPIRATORY ALLERGIC DISEASES AS А PART OF THE PROGRAMME OF STATE GUARANTEES IN THE ORENBURG REGION ^{[№ 1 ' 2013]}
The sources of financing of Territorial programs of government guarantees for providing the citizens of the Russian Federation in Orenburg region free medical care were studied. The technique of formation the List of the most effective and less expensive drugs for therapy of children with bronchial asthma and allergic rhinitis in stationary level was propounded with the purpose of rational use of budget funds.

Zharikova N.V., Zhurenko E.Yu., Korobov V.V., Aysakov T.R., Anisimova L.G., Markusheva T.V.
BIOLOGICAL VARIETY OF BACTERIA – DECOMPOSERS OF CHLORINATED PHENOXYACIDS ^{[№ 6 ' 2009]}

Nenasheva M.N., Korobov V.Yu.
STUDYING OF SPECIES VARIETY OF HYDROBIONS IN CLOSED ECOSYSTEM AT INTERNAL-DRAINAGE SYSTEM OF WATER SUPPLY OF ARTIFICIAL PONDS FOR FISH REARING ^{[№ 6 ' 2009]}

Korobov D.V., Glushchenko Yu.N., Bocharnikov V.N.
QUANTITATIVE CHARACTERISTICS AND PECULIARITIES OF KLOKTUN’S MIGRATION (ANAS FORMOSA) ON KHANKAISKO-RAZDOLNENSK PLAIN (PRIMORSKY KRAI) ^{[№ 10 ' 2007]}
Dynamics of (Anas Formosa) kloktun’s quantity on Khankaisko-Razdolnensk plain during last 35 years is analyzed in this article. Peculiarities of spring and autumn flights of kloktun on the territory of Prikhankaisky lowland and in the valley of the River Razdolnaya are regarded here. Time shift of this duck flight on earlier period, observed last years and weak intensity of autumn flight are indicated in this work.