October 2017, № 9 (209), pages 51–56

doi: 10.25198/1814-6457-209-51

Bekpergenova A.V., Khlopko Y.A., Ivanova E.V., Perunova N.B. FORMATION OF ASSOCIATIONS OF OBLIGATE-ANAEROBIC BACTERIA OF THE GUT HUMANExamination of infection as a form of symbiotic relationship between microorganisms and humans allowed to substantiate the idea of infection as a model system of associative symbiosis. Microsymbiocenosis is one of the vectors of associative symbiosis, where the formation of associations of microorganisms with different types of bonds takes place. A significant contribution to understanding infectious symbiology has been made by new methodological approaches using the persistent potential of microbes in the evaluation of microsymbiocenosis. The study of the mechanisms of intermicrobial interactions has shown that the basis of the formation of microsymbiocenosis is the relationship that determines the nature of the relationship — synergistic and antagonistic.
Since the results of the in vitro experiments revealed that under the action of supernatants, both the increase and decrease in biological properties occur to varying degrees, and there is no change in the indices studied (indifferent effect), to obtain synergistic and antagonistic relationships, the resulting factual material was processed with discriminant analysis, which integrated the investigated properties: growth properties, biofilm formation, antilisozime activity. This made it possible to single out associations with a “synergistic” type of bond: B. bifidum / B. longum; B. longum / E. limosum; E. limosum / B. fragillis; C. perfringens / B. fragilis and “antagonistic” — E. limosum / C. perfringens.
In natural conditions, the vast majority of microorganisms exist in the form of associations, the formation of which gives a certain advantage to members of the microbial community when surviving in various biotopes of the human body. The carried out researches made it possible to establish that in addition to the growth properties in the associations of obligate anaerobic bacteria, there is a change in biological properties (biofilm formation, antilisozime activity) that make up the system-forming factor of microsymbiocenosis, which contributes to understanding the mechanisms of the formation of associative symbiosis of man and can make a difference in the development of microbial compositions of new synbiotics.Key words: obligate-anaerobic bacteria, intermicrobial interactions, antilysozyme activity, the biofilm formation, growth properties, associative symbiosis.

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About this article

Authors: Hlopko Yu.A., Bekpergenova A.V., Ivanova E.V., Perunova N.B.

Year: 2017

doi: 10.25198/1814-6457-209-51

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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