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November 2015, № 13 (188)



Shchepitova N.E., Sycheva M.V., Kartashova O.L. SCRINNING OF ENTEROCOCCUS CULTURE TO CREATE PREPORATION OF PROBIOTIC ACTIONIn the conditions of fight against an antibiotic resistance of microorganisms search of antimicrobic means alternative to traditional antibiotics is of particular importance. Bacteriocins of enterococci can form a basis for new antimicrobic preparations. In this regard screening of new safe strains producers of enterococci for receiving bacteriocins is the actual and perspective direction of modern microbiology. In the specific analysis of bacteria of the genus Enterococcus, defined from intestines of different animals species. In population of faecal enterococci the wide popularity of antagonistic activity regarding bacterias Listeria spp. and Enterococcus spp. which is correlative to the genes of bacteriocinogenia. On the model of experimental infection shows the use the protection effect of listerios defence by antoganistically active enterococci of intestines nature. On the basis of a complex assessment of phenotypic and genetic characteristics of enterococci isolated from intestines of healthy animals is selected strain Enterococcus faecium which is possessing genetic determinants of bacteriocinogenia, the expressed antagonistic effect against Listeria and not having virulence factors.Key words: Enterococcus spp., antagonistic activity, bacteriocin production, factors of virulence, experimental infection, polymerase chain reaction.

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References:

1. Ashmarin, I.P. Statistic methods in microbiology / I.P. Ashmarin, A.A. Vorobyov. — L.: Gos.isd. med. lit. — 1962. — 177 с.

2. Garmasheva, I.L. Identification and taxonomy of enterococci / I.L. Garmasheva, N.K. Kovalenko // Mіkrobіol. Zh. — 2010. — Vol. 72. — No. 5. — P. 49-58.

3. Ermolenko, E.I. Enterococci bacteriocins: problems and prospects. Literature review / E.I. Ermolenko // Vestn. St. Petersburg. Univ. — 2009. — Ser. 11 — Vol. 3. — P. 184-201.

4. Kudlay, D.G. Bakteriotsinogeniya / D.G. Kudlay, V.G. Likhoded. — M.: Medicine, 1966. — 203 p.

5. Litvin, V.Yu. Factors pathogenic bacteria: function in the environment / V.Yu. Litvin, V.N. Pushkareva // Journal of Epidemiology and Microbiology, Immunobiology. — 1994 — Annex. — P. 83-87.

6. Pokhilenko, V.D. Bacteriocins, their biological role and trends of application / V.D. Pokhilenko, V.V. Perelygin // Electronic scientific journal "Investigated in Russia". — 2011. — P. 164-198. URL: http//zhurnal.ape.relarn.ru/articles/2011/016.pdf.

7. Guide to microbiological and virological research methods / ed. M.O. Birger. — M.: Medicine, 1982. — 464 p.

8. Characterization of fsr, a regulator controlling expression of gelatinase and serine protease in Enterococcus faecalis OG1RF / X. Qin, K.V. Singh, G.M. Weinstock [et al.] // J. Bacteriol. — 2001. — Vol. 183. — No. 11. — P. 3372-3382.

9. Contamination of milk by enterococci and coliforms from bovine faeces / D.M. Kagkli, M. Vancanneyt, P. Vandamme [et al.] // J. Appl. Microbiol. — 2007. — Vol. 103. — No. 5. — P. 1393-1405.

10. Detection of bacteriocin production and virulence traits in vancomycin-resistant enterococci of different sources / C. Sabia, S. de Niederhausern, E. Guerrieri [et al.] // Journal of Applied Microbiology. — 2008. — No. 104. — P. 970-979.

11. Development of Multiplex PCR for the detection of asa1, gelE, cylA, esp, and hyl genes in Enterococci and survey for virulence determinants among European hospital isolates of Enterococcus faecium / V. Vankerckhoven, T. Van Autgaerden, C. Vael [et al.] // Journal of Clinical Microbiology. — 2004. — No. 42. — P. 4473-4479.

12. Identification, characterization, and three-dimensional structure of the novel circular bacteriocin, enterocin NKR-5-3B, from Enterococcus faecium / K. Himeno, K.J. Rosengren, T. Inoue [et al.] // Biochemistry. — 2015. — Vol. 54. — No. 31. — P. 4863-4876.

13. Immunomodulatory properties of Enterococcus faecium JWS 833 isolated from duck intestinal tract and suppression of Listeria monocytogenes infection / H.J. Choi, M.S. Shin, S.M. Lee [et al.] // Microbiology and Immunology. — 2012. — Vol. 56. — No. 9. — P. 613-620.

14. Isolation and biochemical characterisation of enterocins produced by enterococci from different sources / M.R. Foulquiй Moreno, R. Callewaert, B. Devreese [et al.] // Journal of Applied Microbiology. — 2003. — No. 94. — P. 214-229.

15. Jackson, C.R. Use of a genus — and species-specific multiplex PCR for identification of enterococci / C.R. Jackson, P.J. Fedorka-Cray, J.B. Barrett // Journal of Clinical Microbiology. — 2004. — Vol. 42. — No. 8. — P. 3558-3565.

16. Lactobacilli and streptococci induce inflammatory chemokine production in human macrophages that stimulates Th1 cell chemotaxis / V. Veckman, M. Miettinen, S. Matikainen [et al.] // J. Leukoc. Biol. — 2003. — Vol. 74. — P. 395-402.

17. Molecular characterization and antibiotic resistance of Enterococcus species from gut microbiota of Chilean Altiplano camelids / K. Guerrero-Olmos, J. Bбez, N. Valenzuela et al. // Infect. Ecol. Epidemiol. [electronic journal]. — 2014. — Vol. 4.

18. Monoassociation with probiotic Lactobacillus delbrueckii UFV-H2b20 stimulates the immune system and protects germfree mice against Listeria monocytogenes infection / L.M. dos Santos, M.M. Santos, H.P. de Souza Silva [et al.] // Med. Microbiol. Immunol. — 2011. — Vol. 200. — No. 1. — P. 29-38.

19. Nigъtovб, K. Bacteriocin-like activity production and resistance in selected enterococci and streptococci of animal origin / K. Nigъtovб, P. Pristas, P. Javorskэ // Archives Animal Nutrition. — 2005. — Vol. 59. — No. 3. — P. 205-211.

20. Occurrence of the structural enterocin A, P, B, L50B genes in enterococci of different origin / V. Strompfovб, A. Laukovб, M. Simonovб [et al.] // Veterinary Microbiology. — 2008. — Vol. 132. — No. 3. — Р. 293-301.

21. Цzdemir, G.B. Phenotypic and genotypic characterization of bacteriocins in enterococcal isolates of different sources / G.B. Цzdemir, E. Oryaşin, H.H. Biyik [et al.] // Indian Journal of Microbiology. — 2011. — Vol. 51. — No. 2. — P. 182-187.

22. Phenotypic and genotypic study of gelatinase and beta-haemolysis activities in faecal enterococci of poultry in Portugal / P. Poeta, D. Costa, N. Klibi [et al.] // J. Vet. Med. — 2006. — Vol. 53. — No. 5. — P. 203-208.

23. Popaganni, M. Ribosomally synthezed peptides with antimicrobial properties: biosynthesis, structure, function, and applications / M. Popaganni // Biotechnol. Adv. — 2003. — Vol. 21. — No. 6. — P. 465-499.

24. Resolution of phenotypically distinct strains of Enterococcus spp. in a complex microbial community using cpn60 universal target sequencing / C.J. Vermette, A.H. Russell, A.R. Desai [et al.] // Microb. Ecol. — 2010. — Vol. 59. — No. 1. — P. 14-24.

25. Safety and technological properties of bacteriocinogenic enterococci isolates from Tunisia / I. Jaouani, M.S. Abbassi, S.C. Ribeiro [et al.] // J. Appl. Microbiol. — 2015. — Vol. 119. — No. 4. — P. 1089-1100.

26. Screening of the enterocin genes and antimicrobial activity against pathogenic bacteria in Enterococcus strains obtained from different origins / W. Theppangna, T. Murase, N. Tokumaru [et al.] // J. Vet. Med. Sci. — 2007. — Vol. 69. — No. 12. — P. 1235-1239.

27. Screening of virulence determinants in Enterococcus faecium strains isolated from breast milk / C. Reviriego, T. Eaton, R. Martнn [et al.] // Journal of Human Lactation. — 2005. — No. 21. — P. 131-137.

28. VanA-type enterococci from humans, animals, and food: species distribution, population structure, Tn1546 typing and location, and virulence determinants / F. Biavasco, G. Foglia, C. Paoletti [et al.] // Applied and Environmental Microbiology. — 2007. — Vol. 73. — No. 10. — P. 3307-3319.

29. Virulence factors in food, clinical and reference enterococci: a common trait in the genus? / T. Semedo, M.A. Santos, M.F. Lopes [et al.] // Systematic and Applied Microbiology. — 2003. — No. 26. — P. 13-22.


About this article

Authors: Sychyova M.V., Shchepitova N.E., Kartashova O.L.

Year: 2015


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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