2015, № 12 (187)

Mushkova I.A., Borsenok S.A., Karimova A.N., Shkandina Y.V. HISTOMORPHOLOGICAL CHANGES IN THE RABBIT CORNEA AFTER IMPLANTATION INTRACORNEAL LENSES AND ELECTRON MICROSCOPIC JUSTIFICATION OF THE RESULTSToday on the international market is approved and available for clinical use different models of intracorneal lenses for surgical correction of presbyopia. Using the technical capabilities and production capacity of domestic manufacturers were modeled and manufactured lenses with geometric parameters, calculated using mathematical modeling taking into account characteristics of the cornea, made of modern polymeric materials. To study the biocompatibility of intracorneal lenses it is necessary to conduct preclinical studies of reactivity of cornea in response to the implantation of the developed products hydroxyethylmethacrylate (HEMA) and olygourethanmethacrylate (OUMA) in an experimental study in vivo and conduction electron-microscopic examination of intact "inlays". When carrying out scanning electron microscopy the surface and edges of intact lenses is rendered smooth surface with no visible chipping. In the study of the shape of the edge surface of intracorneal lenses from HEMA visualized jagged, irregular edge, and lenses from OUMA — smooth, with no visible chipping. When conducting in vivo confocal microscopy in the experimental groups was visualized single collagen fibers, activation of keratocytes, was determined hyperreflexivity and hypocellularity in the area of the lens, pseudokeratinization of the surface epithelium. In the group with the use of GEMA's lenses noted decrease of transparency of the extracellular matrix around the implant. During the light microscope in the experimental groups was visualized bed of inlay. Around bed in both groups was detected activation of cells, presumably native keratocytes. The epithelium and endothelium — no visible changes in the area of inlay and outside this zone. In the control group — no significant changes in all layers of the cornea. Thus, in the course of the study it was found that the developed products do not cause pronounced cellular and tissue response of the cornea of the rabbit, the rendered methods of in vivo confocal microscopy and the light microscopy. However, jagged, irregular shape of the edge surface of GEMA's lenses caused, apparently, more technically complicated manufacturing technology ("turning") can cause greater activation of cells around the bed of the cornea and a moderate seal of stroma around the lens, in comparison with OUMA's lenses.Key words: inlay, light microscopy, in vivo confocal microscopy, electron scanning microscopy, presbyopia.

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References:

1. Arlt E, Krall E, Moussa S, Grabner G, Dexl A. Implantable inlay devices for presbyopia: the evidence to date // Clinical ophthalmology. — 2015 Jan 14;9:129-37

2. Baily C, Kohnen T, O'Keefe M. Preloaded refractive-addition corneal inlay to compensate for presbyopia implanted using a femtosecond laser: one-year visual outcomes and safety // Journal of Cataract and Refractive Surgery. — 2014. — Vol. 40 — Issue 8 — P. 1341–1348

3. Barraquer J.I., Gomes M.L. Permalens hydrogel intracorneal lenses for spherical ametropia // J. Refract. Surg. — 1997. — Vol. 13. — P. 342-348

4. Choyce D.P., Horgan S.E., Fraser S.G., Alexander W.L. Twelve year follow-up of unfenestrated polysulfone intracorneal lenses in human sighted eyes. // Journal of Cataract and Refractive Surgery. — 1996. — Vol. 22 — Issue 8 — P. 1045-1051

5. Keates RH, Martines E, Tennen DG, Reich C. Small-diameter corneal inlay in presbyopic or pseudophakic patients // Journal of Cataract and Refractive Surgery. — 1995. — Vol. 21 — Issue 5 — P. 519-521

6. McCarey et. al. Hydrogel implants for refractive keratoplasty: corneal morphology // Cur. Eye Res. — 1982/83. — Vol. 2. — P. 29-38.

7. Lindstrom R.L., Macrae S.M., Pepose J.S., Hoopes P.C. Corneal inlays for presbyopia correction // Curr. Opin. Ophthalmol. — 2013. — Vol. 24. — №4 — Р. 281-287

8. Pinsky P.M. Three-dimensional modeling of metabolic species transport in the cornea with a hydrogel intrastromal inlay // Invest. Ophthalmol. Vis. Sci. — 2014. — Vol. 55. — № 5. — Р. 3093-3106

9. Seiler T., Colin J., Ferrara P., Alio J. New approaches for corneal remodeling in refractive surgery // Eurotimes. — 2005, Aug. — P. 115-121.

About this article

Authors: Borzenok S.A., Shkandina Yu.V., Mushkova I.A., Karimova A.N.

Year: 2015

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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