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2013, № 4 (153)



Belyy Yu.A, Temnov A.A., Mirgorodskaya S.A. THE DEVELOPMENT OF CULTIVATION TECHNOLOGY OF MESENCHYMAL STEM CELLS WITH MAGNETIC PARTICLES FOR THE SUBRETINAL INJECTIONThe technology of the cultivation of mesenchymal stem cells with magnetic particles for the subretinal injection was developed in this work. The estimation of the effect of magnetic particles on the proliferation and functional activity of mesenchymal stem cells has been done. We studied the induction of apoptosis and viability of stem cells with magnetic particles. It has been proved that the technology is safe, the efficiency of the labeling of MSCs by magnetic particles for this technology is about 90%, the viability of stem cells is about 95%.Key words: stem cells, magnetic particles, subretinal injection, fixation.

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References:

1. Aramant R.B., Seiler M.J. Progress in retinal sheet transplantation // Prog. Retin. Eye Res. — 2004. — V. 23, N 5. — P. 475-494.

2. Arbab AS, Jordan EK, Wilson LB, Yocum GT, Lewis BK, Frank JA. In vivo trafficking and targeted delivery of magnetically labeled stem cells. Hum Gene Ther. — 2004 — Apr;15(4) — V. 351-60.

3. Arragval S., Pittenger M.F. Human mesenchymal stem cells modulate allogeneic immune cell responses // Blood. — 2005 — V. 105. — P. 1815 — 1822.

4. Bartholomew A., Sturgeon C., Siatskas M. et al. Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo // Exp. Hematol. — 2002. — V.30. — P. 42-48.

5. Kyrtatos P.G. et al. Magnetic Tagging Increases Delivery of Circulating Progenitors in Vascular Injury. // JACC Cardiovascular Interventions. — 2009 — 2 (8) — P. 794.

6. Lazarus H.M., Koc O.N., Devine S.M. et al., Cotransplantation of HLA-identical sibling culture-expanded mezenchymal stem cells and hematopoetic stem cells in hematologic malignancy patients // Biol Blood Marrow Transplant. — 2005. — №11. — V.5. — P.389-398.

7. Mimeault M, Batra SK: Concise review: recent advances on the significance of stem cells in tissue regeneration and cancer therapies. Stem Cells — 2006 — 24:2319-2345.

8. Noort WA, Krusselbrink AB, in"t Anker PS et al. Mesenchymal stem cells promote engraftment of human umbilical cord blood-derived CD34(+) cells in NOD/SCID mice//Exp Hematol/ — 2002. — V. 30. — P. 870-878.

9. Sekiya I., Larson B., Smith J. et al. Expansion of human adult stem cells from bone narrow stroma: conditions that maximize the yields of early progenitors and evaluate their quality. // Stem Cells. — 2002. — V. 20. — P. 530 — 541.

10. Silverman M.S., Hughes S.E. Photoreceptor transplantation in inherited and environmentally induced retinal degeneration: anatomy, immunohistochemistry and function. // Prog. Clin. Biol. Res. — 1989 — V. 314. — P. 687 — 704.

11. Takahashi M, Palmer TD, Takahashi J, Gage F.H. Widespread integration and survival of adult-derived neural progenitor cells in the developing optic retina // Mol Cell Neurosci — 1998 V 12. — N 6. — P.340-348.

12. Belyy Yu.A., Tereshchenko A.V., Konoplyannikov A.G., Soloviev D.K. The method of treatment of atrophy of the optic nerve of different etiology// 2008 — patent №2375019

13. Belyakovsky P.V., Poznyak N. I. Lobanok E.S. Influence of stromal cells on manifestation of a kainantny optikoneyropatiya // Ophthalmology in Belarus — 2009 — №1 — P. 91-96

14. Kuprashvili I.T. Experimental studying of efficiency of cellular transplantation at post-traumatic pathology of a retina // Dissertation of the candidate of medical sciences. — М., 2009. — 25 p.

15. Sergeev V.S. Immunological properties of multipotent progenitor of mesenchymal stromal cells // Cellular transplantation and Tissue Engineering. — 2005. — №2 — P.39-42.

16. Tahchidi H.P, Gavrilova N.A., Komova O.Y., Lanevskaya N.I., Sabourin I.N., Sergeev S.A., Pavlova, G.V., Revischin A.V/, Orlov O. Y., Bastakov V.A. The impact of stromal/progenitor cells on the functional condition ahd the degree of severity of degenerative changes of retina in rats line Campbell // Ophthalmosurgery — 2010 — №3 — Р. 33-38.


About this article

Authors: Belyy Yu.A., Temnov A.A., Mirgorodskaya S.A.

Year: 2013


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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