Temnov A.A., Belyj Yu.A., Mirgorodskaya S.A., Semenov A.D., Revishchin A.V., Pavlova G.V., Kust N.N.
EXPERIMENTAL STUDY OF THE LOCAL SUBRETINAL INJECTION XENOGENIC STEM CELLS LABELED WITH MAGNETIC PARTICLES ^{[№ 12 ' 2014]}
There has been developed a technique for local subretinal injection of xenogeneic stem cells labeled with magnetic particles and to prove experimentally its effectiveness. In the experimental group we used original complex of polymer elastic magnetic implant (PEMI) with laser probe, then we made a median vitrectomy and injected HEK-293 GFP under the retina using a specially designed dispenser. In the control group PEMI was not fixed. Results. The morphological study results showed that cells were located in the subretinal space up to 14 days in the experimental group, and only up 3 days in the control group. Conclusion. The suggested surgical technique enables to control the injection of cells into the subretinal space, reduces the risk of tissue damage and exit cells in the vitreous space.

Belyy Yu.A, Temnov A.A., Mirgorodskaya S.A.
THE DEVELOPMENT OF CULTIVATION TECHNOLOGY OF MESENCHYMAL STEM CELLS WITH MAGNETIC PARTICLES FOR THE SUBRETINAL INJECTION ^{[№ 4 ' 2013]}
The technology of the cultivation of mesenchymal stem cells with magnetic particles for the subretinal injection was developed in this work. The estimation of the effect of magnetic particles on the proliferation and functional activity of mesenchymal stem cells has been done. We studied the induction of apoptosis and viability of stem cells with magnetic particles. It has been proved that the technology is safe, the efficiency of the labeling of MSCs by magnetic particles for this technology is about 90%, the viability of stem cells is about 95%.