Sutormin.O.S., Sukovataya I.E., Kratasyuk V.A.
THE STABILIZING EFFECT OF GLYCEROL AND SUCROSE ON THE COUPLED ENZYME SYSTEM OF BIOLUMINESCENCE BACTERIA NADH:FMN-OXIDOREDUCTASE-LUCIFERASE ^{[№ 10 ' 2013]}
The influence of the viscosity of the reaction medium on the thermal stability and thermal inactivation kinetics of the coupled enzyme system of bioluminescence bacteria NAD(P)H:FMN-oxidoreductase-luciferase was investigated. It was showed that the increasing viscosity of the reaction medium leads to increase of thermal stability of the coupled enzyme system. Sucrose stabilizes long-lived intermediate of bioluminescent reaction.