Shelamova S.А., Tyrsin Y.А.
THE ROLE OF CARBOXYL GROUPS IN THE ACTIVITY OF LIPASE I RHIZOPUS ORYZAE 1403 ^{[№ 4 ' 2012]}
Participation of carboxyl groups in catalytic action Lipase I Rhizopus oryzae 1403 is proved inactivated carbodiimide (EDC) at presence nucleophile — glycine ethyl ester. The amino acid analysis modified enzyme has excluded an opportunity reacted with EDC tyrosine and histidine, and absence of restoration of activity hydrocxylamine — with serine. At practically complete loss of activity enzyme was modified 11 carboxyl groups; under condition of the pseudo-first order of reaction hydrolysis — three. Research of kinetic hydrolysis triolein and tributirin have shown, that V_max differed on both a little substrates, and K_M on tributirin has increased in 1,47, and on triolein — in 1,18 times. It results in a conclusion, that of carboxyl group answer for creation active conformation enzyme at linkage with certain substrate.

Shelamova S.A., Tyrsin Yu.A.
INDUCTION OF BIOSYNTHESIS OF LIPASE BY MICROMICETES ^{[№ 1 ' 2012]}
Biosynthesis of lipazy Rh oryzae 1403is induced by triglycerides, fat acids and their derivatives — twins. The highest effect was produced by olive oil, twin 80 and oleic acid. The producer is able to synthesize the two produce lipolytic enzymes, molecular weight was the same of both isoenzymes, and was 44±2 kDa. Between izoformsi there was great satisfaction in amino acid content.