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2012, № 4 (140)



Shelamova S.А., Tyrsin Y.А. THE ROLE OF CARBOXYL GROUPS IN THE ACTIVITY OF LIPASE I RHIZOPUS ORYZAE 1403 Participation of carboxyl groups in catalytic action Lipase I Rhizopus oryzae 1403 is proved inactivated carbodiimide (EDC) at presence nucleophile — glycine ethyl ester. The amino acid analysis modified enzyme has excluded an opportunity reacted with EDC tyrosine and histidine, and absence of restoration of activity hydrocxylamine — with serine. At practically complete loss of activity enzyme was modified 11 carboxyl groups; under condition of the pseudo-first order of reaction hydrolysis — three. Research of kinetic hydrolysis triolein and tributirin have shown, that Vmax differed on both a little substrates, and KM on tributirin has increased in 1,47, and on triolein — in 1,18 times. It results in a conclusion, that of carboxyl group answer for creation active conformation enzyme at linkage with certain substrate.Key words: lipase, Rhizopus, carboxyl group.

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About this article

Authors: Shelamova S.A., Tyrsin Yu.A.

Year: 2012


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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