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2013, № 6 (155)



Ali F.M., El-Khatib A.M., Sabry S.A., Abo-Neima S.E., Motaweh H.A. CONTROL OF STAPHYLOCOCCUS AUREUS GROWTH BY ELECTROMAGNETIC THERAPYThe present work aimed to study the biological effects of Extremely Low Frequency Electromagnetic Field (ELF-EMF) on the growth rate, morphology and antibiotic sensitivity patterns of the Gram-positive bacterium Staphylococcus aureus to determine any morphological and metabolic changes that might have been caused by ELF-EMF. In order to compare cell viability, number of colony-forming units (CFU) and growth rate (optical density at 600 nm) was determined. The results showed that a highly significant inhibition effect occurred when S.aureus was exposed to resonance of 0.8 Hz QAMW for a single exposure (120 min). Moreover, exposed cells became more sensitive to the tested antibiotics compared to control. Significant ultra structural changes occurred as observed by Transmission Electron Microscope (TEM). Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) revealed genetic fingerprinting variation as observed in the electrophoresis patterns between exposed and unexposed cells of S.aureus.Key words: Staphylococcus aureus, ELF-EMF, bacterial growth rate, antibiotic sensitivity, morphological changes, TEM, RAPD-PCR.

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References:

1. C. Crassi,M.D Ascenzo, A. Torsello et al, [2004] Effects of electromagnetic fields on voltage-gated Ca2+ channels and their role in modulation of neuroendocrine cell proliferation and death cell calcium,vol.35,No.4,pp.307-315.

2. A. Foletti,A.Lisi,M.Ledda,F.Decarlo,andS.Grimaldi[2009] Cellular ELF signals as a possible tool in informative medicine, Electromagnetic Biology and Medicine, vol. 28, no.1, pp. 71-79.

3. R. Goodman and M.Blank, [1998] Magnetic field stress induces expression of hsp 70, Cell stress and chaperones, vol.3, No. 2, pp. 79-88.

4. R. Goodman,A. Lin-Ye,M.S. Geddis et al, [2009] Extremely low frequency electromagnetic fields activate the ERK cascade, increase hsp 70 protein level and promote regeneration in planaria, International Journal of Radiation Biology, vol. 85, No. 10, pp. 851-859.

5. F. Bersani, F. Marinelli, A. Ognibene et al, [1997] Intramembrane protein distribution in cell cultures is affected by 50 Hz pulsed magnetic fields electromagnetics, vol. 18, No. 7, pp. 463-469.

6. M. de Mattei, K. Varani, F.F. Masieri et al. [2009] Adenosine analogs and electromagnetic fields inhibit prostaglandin E2 release in bovine synovial fibroblasts, Osteoarthritis and cartilage, vol. 17, No. 2, pp. 252-262.

7. F. Tian, T. Nakahara, K. Wake, M. Taki, and J. Miyakoshi, [2002] Exposure to 2.45 GHz electromagnetic fields induced hsp 70 at a high SAR of more than 20 W/Kg in human glima MO54 CELLS, International Journal of Radiation Biology, vol. 78, No. 5, pp. 433-440.

8. M.T. Santini,A.Ferrante,R.Romano et al, [2005] A 700MHz 1H-NMR study reveals apoptosis-like behavior in human K562 erythroleukemic cells exposed to a 50 Hz sinusoidal magnetic field, International Journal of radiation Biology, vol. 81, No. 2, pp. 97-113.

9. A. Lisi, M. Ledda, E. Rosola et al. [2006]Extremely low frequency electromagnetic field exposure promotes differentiation of pituitary corticotrope-derived At T20 D16V cells, electromagnetics, vol. 27, No. 8, pp. 641-651.

10. R.Piacentini,C.Ripoli,D.Mezzogori,G.B.Azzena,and C.Grassi, [2008] Extremely low– frequency electromagnetic fields promote in vitro neurogenesis via upregulation of cavl-channel activity,Journal of cellular physiology, vol. 215, No. 1, pp.129-139.

11. J.Zhou.G.Yao,J. Zhang, and Z.Chang, and Z. Chang, [2002]CREB DNA binding activation by a 50Hz magnetic field in HL60 cells is dependent on extra-and intracellular Ca2+ but not PKA,PKC,ERK, or p38 MAPK, Biochemical and Biophysical Research Communications, vol. 296, No. 4, pp. 1013-1018.

12. R.Iorio,S.DelleMonache,F.Bennato et al. [2011] Involvement of mitochondrial activity in mediating ELF-EMF stimulatory effect on human sperm motility, Bioelectromagnetics, vol. 32, No. 1, pp. 15-27.

13. C.Morabito,F.Rovetta,M.Bizzarri,G.Mazzoleni,G.Fano,and M.A.Mariggio, [2010]Modulation of redox status and calcium handling by extremely low frequency electromagnetic fields in C2C12 muscle cells: a real — time,single-cell approach,Free Radical Biology and Medicine, vol. 48, No. 4, pp. 579-589.

14. K.Zwirska-Korczala,J.Jochem,M.Adamczyk-sowa et al, [2005] Effects of extremely low frequency electromagnetic fields on cell proliferation, antioxidative enzyme activities and lipid peroxidation in 3T3-L1 preadipocytes an invitrostudyJournal of physiology and pharmacology, vol. 56, No. 6, pp. 101-108.

15. S.DiLoreto,S.Falone,V.Caracciolo et al, [2009] Fifty hertz extremely low frequency magnetic field exposure elicits redox and trophic response in rat-cortical neurons, Journal of cellular physiology, vol. 219, No. 2, pp. 334-343.

16. Fadel, M.A., S.M. Wael, R. Mostafa, [2003] Effect of 50Hz, 0.2 mT magnetic fields on RBC properties and heart functions of albino rats, Bioelectromagnetic, vol.24,No.1,pp.535-545.

17. Y.D.Alipov,I.Y.Belyaev, and O.A.Aizenberg, [1994] Systemic reaction of Escherichia coli cells to weak electromagnetic fields of extremely low frequency, Bioelectrochemistry and Bioenergetics,vol.34,No.1,pp.5-12.

18. A.Inhan-Garip,B.Aksu,Z.Akan,D.akakin,A.N.ozaydin, and T.San, [2008]Effects of extremely low frequency electromagnetic fields on growth rate and morphology of bacteria,International Journal of Radiation Biology vol,87,No.12,pp.1155-1161.

19. L.Strasak,V.Vetterl, and J.Smarda, [2002] Effects of low frequency magnetic fields on bacteria Escherichia coli, Bioelectrochemistry,vol.55,No.1-2,pp.161-164.

20. L.Fojt,L.Strasak,V.Vetterl,and J.Smarda, [2004] Comparison of the low frequency magnetic field effects on bacteria Escherichia coli,Leclerciaadecarboxylata and staphylococcuaaureus, Bioelectrochemistry,vol.63,No.1-2,pp.337-341.

21. O.R.Justo,V.H.Perez,D.c.Alvarez, and R.M.Alegre, [2006] Growth of Escherichia coli under extremely low-frequency electromagnetic fields Applied Biochemistry and Biotechnology,vol.134,No.2,pp.155-163.

22. E.S. A.Gaafar, M.S. Hanafy, E.T. Tohamy, and M.H.Ibrahim, [2006] Stimulation and control of E.Coli by using an extremely low frequency magnetic field.Romanian Journal of Biophysics, vol.16, No.4, pp.283-296.

23. I.V.Babushkina,V.B. Borodulin,N.A.Shmetkova et al, [2005] The influence of alternating magnetic field on Escherichia coli bacterial cells, Pharmaceutical Chemistry Journal,vol.39,no.8,pp.398-400.

24. L.Cellini,R.Grande,E.DiCampli et al, [2008] Bacterial response to the exposure of 50Hz electromagnetic fields, Bioelectromagnetics,vol.29,No.4,pp.302-311.

25. I.Belyaev, [2011] Toxicity and SOS-response to ELF magnetic fields and nalidixic acid in E.coli cells, Mutation Research,vol.722,No.1,pp.56-61.

26. JayaramS.,Castle G.S. And Margaitis,A Biotech Bioeng, [1992] vol.40,No.1,pp.1412-1420.

27. Pothakamury U.,Monsalve-Gonzalez A.,Barbosa-Canovas G. and Swanson B, [1996] Journal.food prot.vol.59:1167-1171.

28. Jeantet R.,Baron F.,Nau F.,Rorgnant M. and Brule G. Journal.foodprot,[2000] vol.62,No.12,pp.1381-1386.

29. Alvarez I., Raso J.,PalopA.andSalaF, [2000] Influence of different factors on the inactivation of salmonella senftienberg by pulsed electric fields. International.Journal.Food Microbial. vol.13,No.1,pp.16-34.

30. Amiali M., Ngadi M., Smith J.andRaghavanV, [2007] Synergistic effect of temperature and pulsed electric field on inactivation of Escherichia coli O157:H7 and Salmonella enteritidis in liquid egg Yolk. Journal Food Eng. vol.79, No.1, pp.689-694.

31. JiW.,HuangH.,DengA.and Pan C, [2009] Effects of static magnetic fields on Escherichia coli.Micron. vol.40,No.1,pp.894-898.

32. Tagourti J., El May A.,Aloui A.,Chatti A.,AissaR.andLandoudoulsi A, [2010] Static magnetic field increases the sensitivity of Salmonella to gentamicin Ann Microbiol.vol.60,no.1,pp.519-522.

33. FadelM.Ali, ReemH.ElgebalyA.Ali,FakhryF.Ibrahim, [2005] Control of Ehrlich tumor growth in mice by electromagnetic waves at resonance frequency,in vivo, Electromagnetic biology and medicine, vol.24,No.1,pp.9-12.

34. El Hag,M, [2003] Effect of extremely low frequency electric fields on some biophysical and biological activity of some microorganisms, PHD thesis Faculty of Science, Cairo University.

35. FadelM.Ali,M.A.Ahmed and M.A.El Hag, [2009] Control of sclerotiumcepivorum (Allium White Rot) activities by electromagnetic waves at resonance frequency. Australian Journal of Basic and Applied Science,issn 1991-8178,vol.3,No.3,pp.1994-2000.

36. Aronsson,K.,U.Ronner and E.Borch, [2005] permeabilization and subsequent leakage of intracellular compounds due to pulsed electric field processing International.Journal. Food Microbial.,vol.99,No.1,pp.19-32.

37. Elez-Martinez,P.J.Escola-Hernandez,R.C.Soliva-Fortuny and O.Martin-Belloso, [2004] Inactivation of Saccharomyces cerevisiae suspended in orange juice using high-intensity pulsed electric fields.J.Foodprot., vol.67,No.11,pp.2596-2602.

38. Obermeier A., Matl F., Friess, [2009] Inactiviation of Escherichia coli, Listeria innocua and saccharomyces ceevisiae in relation to membrane W. and Stemberger A. Growth inhibition of staphylococcus aureus induced by low–frequency electric and electromagnetic fields. Bioelectromagnetics. vol.30,No.1,pp.270-279.

39. Atlas R, [1995] Principles of Microbiology Mosby-Year book,INC.

40. AyseInhan-GaripZafer Akan, BurakAksu, DilekAAkakin, NiluferOzaydin, TangulSan, [2011]’’Effect of extremely low frequency electromagnetic fields on growth rate and morphology of bacteriaInternational Journal of Radiation Biology, vol. 87.no.12.pp.1155–1161.

41. Neumann B,Pospiech A, S chairrer HU, [1992]. Rapid isolation of genomic DNA from gram negative bacteria. Trends. 8:332-333.

42. Hai-Rong chheng & Ning, Jiang, [2006]. Extremely Rapid extraction of DNA from bacteria and yeasts Biotechnology letters vol (28): pp55-59.

43. Martinez,V.Alonso,A.Quispe,M.C.Thqmas,R.Alonso,J.E.Pineroi,.C.Gonzalezi,A.Ortega and B.Valladares, [2003] RAPD method useful for distinguishing Leishmaniaspecies design of specific primers for L. braziliensis.

44. Williams,J.G.,Kubelik,A.R.,Livak,K.J.,RafalskiI,J.A.&Tingeey,S.V, [1990] DNA polymorphins amplified by arbitrary primers are useful as genetic markers.Nucleic Acids Research18, 6531–6535.

45. Padmalatha K,Prasad M.N.V, [2006] Optimization of DNA isolation and PCR protocol for RAPD analysis of selected medicinal and aromatic plants of conservation concern from Peninsular India African Journal of Biotechnology vol. 5. No3, pp.230-234.

46. Kamaledin Eltoum, Imadeldin Aradaib, and Suliman El-Sanousi., (2003) PCR-based randomly amplified polymorphic DNA(RAPD) fingerprinting for detection of genetic diversity among sudanese isolates of Haemophilus somnus Veterinarski arhiv Journal Vol. 73(6), PP.315-321.

47. Fadel, M.A,(1998)A new metabolic biomagnetic resonance model to describe the interaction of ELF-EM field with biological system,International school on Theoretical Biophysics,Moscow.

48. Fadel Mohamed Ali,Azza Mahmoud Gawish,Massarat Bakr Saddek Osman, Ashraf Mahmoud Abdelbacki, and Amira Hamdi El-Sharkawy.(2012) Control of Salmonella activity in rats by pulsed ELF Magnetic field (in vivo study). Journal of international Dental and Medical Research, Vol 5,No (2),pp.129-135.

49. Klachkova, Y.F., M.E. Kvitkina, [1993] Disinfecting cattle slurry by treatment with electromagnetic radiation, Problemy Veterinarnoi Sanitarii I., Ekologii,,vol 2, pp.14–18.

50. Stansell, M.J., W.D. Winters, R.H. Doe, B.K. Dart, [2001] Increased antibiotic resistance of E. coil exposed to static magnetic field, Bioelectromagnetics,, vol 22,No2, pp.129–137.

51. Yenugu S, Hamil KG, Radhakrishnan Y, French FS, Hall SH:TheAndrogen Regulated Epididymal Sperm-Binding Protein,ESC42, Is An Antimicrobial Beta-Defensin, [2004]. Endocrinology,vol.145.pp.3165-3173.

52. Friedrich, W., Galensa, R., Geisler, R. and Grlich, R, [2000] HPLC analysis of polyphenols withcoulometric electrode array detector. Eur. Comm., [Rep.] EUR, EUR 18169, Polyphenols in food.Vol 25. No 32. PP.2832:2855.

53. Rodriguez, A. B., J. B. Anderson, F. P. Siringan, and M. Taviani, [2004] Holocene evolution of the east Texas coast and inner continental shelf: Along-strike valiability in coastal retreat ratesJournal of Sedimentary Research, vol. 74, No, p. 405-421.

54. Ayse Inhan-Garip, Burak Aksu,Zafer Akan, Dilek Akakin, A. Nilufer Ozaydin & Tangul San.,(2011) Effects of extremely low frequency electromagnetic fields on growth rate and morphology of bacteria International Journal of Radiation Biology, vol. 87. No12,pp. 1155-1161.

55. Jaegu C.,DouyanW.,Takao N.,Sunao K.,Hidenori A., XiaofeiL.,Hiroshi S.,HarumichiS.,Hitoshi M.A.,XiaofeiL.,Hiroshi S.,Harumichi S., Hitoshi M. and Takeshi S, [2008] Inactivation of spores using pulsed electric field in a pressurized flow system. J.App.Phys. vol.104,No.1,pp.094701-094701.

56. Ruiz-Gomez M.,Sendra-PorteroF.and Martinez-Morillo M, [2010] Effects of 2.45 Mt sinusoidal 50 Hz magnetic field on Saccharomyces cerevisiae strains deficient in DNA strand breaks repair. International.Journal.Radiat.Biol.vol.86, No.1, pp.602-611.


About this article

Authors: Motaweh N.A., Sabru S.A., Ali F.M., El-Khatib A.M., Abo-Neima S.E.

Year: 2013


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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