Parkhomenko A.N., Stognieva А.А.
EVALUATION OF THE STATE OF SOILS USING MICROBIOLOGICAL INDICATORS IN THE ANTHROPOGENIC IMPACT
Within the rising technogenic impact on the environment, the mixed pollution, for example, with the different oil products, sulphur pollution can be frequently observed. Such an impact on the microorganisms is very inconsiderable. At the same time, it is known that the bioagent on basis of indigenous microorganisms can reduce the pollution. The isolation and study of the properties of such cultures is promising. The article presents data on the study of the microorganisms, isolated from soils in different conditions anthropogenic impact. Object of research is the soil samples, taken at experimental sites of the Astrakhan gas processing plant, oil and sulphur polluted. The effect of oil products and sulphur pollution on the number of saprotrophic, oligotrophic and hydrocarbon oxidizing microorganisms in soil were studied in field conditions. It is shown that high number of hydrocarbon oxidizing microorganisms reflects degree of impact of pollution at all stages of the experiment. The soils under oil products and sulphur pollution had four hundred and forty amounts of hydrocarbon oxidizing microorganisms in the first month after pollution. It is shown that the isolated microorganisms possess high hydrocarbon oxidizing and emulsifying activity against oil, kerosene and engine oil. The highest index of emulsification is shown against oil and engine oil (92 and 95 %). This could indicate a high adaptation and destructive activity of microorganisms. Obtained results testify also possibility to develop on basis of isolated cultures a bioagent for rehabilitation of soil, polluted by oil, oil products and sulphur.
Key words: soil, oil pollution, hydrocarbon oxidizing microorganisms, emulsifying activity, bioagent.
1. Andreanov V.A. Geoecological aspects of the actions of the Astrakhan gas complexes. Astrakhan: Publication of AGMA. 2002. 245 p.
2. Bekturova A., Masalimov Zh.K. et al. The emulsifying activity of certain hydrocarbon— degrading miroorganisms // KazNU Bulletin. Biology series. — 2013. — №3/1 (59). — P. 56-58.
3. Zvyagintsev D.G. Biological activity of soils and some scales for its evaluation // Soil Science. — 1978. — №6. — P. 48-54.
4. Kaiyrmanova G.K., Zhubanova A.A., Yernazarova A.K. et al. Reciept microbial associations used for of bioremediation of oik wastes of deposit “Janajol” // KazNU Bulletin. Biology series. — 2014. — №1/2 (60). — P. 240-243.
5. Kireeva N.A., Vodopyanov V.V., Miftakhova A.M. Effect of oil pollution on cellulase activity of soils // Soil Science. — 2000. — №6. — P. 748-753.
6. Kireeva N.A., Vodopyanov V.V., Miphtakhova A.M. Biological activity of oil contaminated soils. Ufa: Hilem. 2001. 376 p.
7. Practicals on microbiology: book for students of higher learning institutions / edited by A.I. Netrusov. M.: Academia. 2005. 608 p.
8. Saksonov M.N., Abalakov A.D., Dan’ko L.V. Ecological monitoring of oil and gas industry. Physico-chemical and biological methods. Irkutsk: Publ. house of IU. 2007. 114 p.
9. Yazikov Ye.G., Shatilov A.Yu. Geoecological monitoring. Textbook. Tomsk. 2003. 336 p.
10. Cooper D.G., Goldenberg B.G. Surface active agents from two Bacillus species // Appl.Environ.Microbiol. — 1987. — Vol.53, № 2. — P. 224-229.
11. Ganesh A. and Lin J. Diesel degradation and biosurfactant production by Gram-positive isolates // African Journal of Biotechnology. — 2009. — V.8 (21). — P. 5847-5854.
12. Jaysree R.C., Subham B., Priyanka P.S. Isolation of biosurfactant producing bacteria from environmental samples // Pharmacology online.— 2011. — V. 63. — P. 1427-1433.
13. Kosaric N. Biosurfactants and theirapplication for soil bioremediation // Food Technol. Biotechnol. — 2001. — V.39. — P. 295-304.
14. Mulligan C.N., Yong R.N., Gibbs B.F. Surfactant-enhanced remediationof contaminated soil // Engineering Geology. — 2001. — V.60. — P. 371-380.
15. Zhang Y., Miller R.M. Effect of rhamnolipid (biosurfactant) on solubization and biodegradation of n-alkanes // Appl. and Environ. Microbiol. — 1995. — Vol.61. — P. 2247-2251.
About this article
Authors: Parkhomenko A.N., Stognieva А.А.