October 2017, № 9 (209), pages 47–50

doi: 10.25198/1814-6457-209-47

Balkin A.S., Cherkasov S.V. DEVELOPMENT OF COCULTIVATION MODEL OF FREE-LIVING AMOEBA ACANTHAMOEBA CASTELLANII WITH BACTERIA SALMONELLA ENTERICA SEROVAR TYPHIMURIUM 14028SAbility of salmonella to persist and replicate within free-living amoebae allows bacteria to survive adverse environmental conditions. However, many aspects related to the survival of salmonella in the environment and the interactions with eukaryotes remain poorly understood.
In this study, the interaction of Salmonella enterica serovar Typhimurium 14028S with cells of free-living amoebas of Acanthamoeba castellanii was studied. Established, that bacteria of this strain are able to penetrate and survive inside A. castellanii phagosome. Most of salmonella is absorbed by amoebas during the first hour of cocultivation. Cytotoxic effect of salmonella is observed with respect to acanthamoeba after 8 hours of co-cultivation and reaches a maximum after 16 hours.
Developed model of interaction between Salmonella and Acanthamoeba is promising for studying the survival strategy of pathogenic microorganisms in environmental conditions. This model can be used to evaluate expression of salmonella genes during phagocytosis by protozoa.Key words: Salmonella, Acanthamoeba, virulence, phagocytosis.

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About this article

Authors: Balkin A.S., Cherkasov S.V.

Year: 2017

doi: 10.25198/1814-6457-209-47

Editor-in-chief

Sergey Aleksandrovich
MIROSHNIKOV

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