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2013, № 10 (159)



Esimbekova E.N., Rimatskaya N.V., Sukovataya I.E., Kratasyuk V.A. BIOLUMINESCENT RAPID METHOD FOR ESTIMATION OF INTEGRAL WATER TOXICITY AND AIR POLLUTIONThe bioluminescent rapid method was developed to estimate the integral toxicity of natural and wastewater. Sensitivity analysis of immobilized bioluminescent reagent to samples with different air pollution carried out.Key words: Ecological monitoring. Bioluminescence. The coupled enzyme system of bioluminescence bacteria NAD(P)H:FMN-oxidoreductase–luciferase, Immobilization, Integral water toxicity, Air pollution.

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References:

1. Gonzalez, C., Greenwood, R., & Quevauviller, P. (Eds.). (2009) Rapid chemical and biological techniques for water monitoring. Chicester: Wiley.

2. Kratasyuk, V.A., Esimbekova E.N., Gladyshev M.I., Khromichek E.B., Kuznetsov A.M., Ivanova E.A. The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems // Chemosphere, 2001. V.42, N 8. P. 909-915.

3. Vetrova E., Esimbekova E., Remmel N., Kotova S., Beloskov N., Kratasyuk V., Gitelson I. A bioluminescent signal system: detection of chemical toxicants in water // Luminescence, 2007. V.22, N 3. P. 206 — 214.

4. Kratasyuk V.A., Esimbekova E.N. (2005) Method for production of immobilized multicomponent reagent for bioluminescent analysis. Invention Patent N 2252963.

5. Esimbekova, E. N., Torgashina, I. G., & Kratasyuk, V. A. (2009). Comparative study of immobilized and soluble NADH:FMN-oxidoreductase–luciferase coupled enzyme system. Biochemistry (Moscow), 74(6), 695–700.

6. Kudryasheva, N. S., Zyuzikova, Y. V., Gutnik, T. V., & Kuznetsov, A. M. (1996). Effect of metal salts on bioluminescent systems of differing complexity. Biophysics (Moscow), 41 (6), 1287–1292.

7. Deryabin, D. G., & Aleshina, E. S. (2008). Effect of salts on luminescence of natural and recombinant luminescent bacterial biosensors. Applied Biochemistry and Microbiology (Moscow), 44(3), 292–296.


About this article

Authors: Esimbekova E.N., Rimatskaya N.V., Sukovataya I.E., Kratasyuk V.A.

Year: 2013


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

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