Vershinin A.A., Petrov A.M., Zaynulgabidinov E.R., Karimullin L.K.
OIL POLLUTION AND MICROBIAL BIOMASS IN DIFFERENT TYPES OF SOILS ^{[№ 3 ' 2018]}
Content of microbial biomass carbon (Cmic) in light-grey forest medium loamy soil, grey forest light loamy soil, dark-grey forest light loamy soil, sod-podzolic sandy loamy soil and sod-podzolic medium loamy soil under different levels of residual content of oil products was studied. The nature of change of microbe biomass carbon and share of microbial carbon in organic substance of soil (Cmic/Corg) dependent on soil type, granulometric composition and residual content of oil. Increase of non-polar hydrocarbons concentration led to decrease both microbial biomass carbon and Cmic/Corg. Among the soils studied microbiota of sod-podzolic sabulous soil appeared to be the most sensitive to oil pollution. Carbone content of dark-grey forest light loamy soil remained at high level and virtually unchanged even high doses of pollutant. The lack of inhibitory effect on the basal respiration of studied soils within the using range of pollutant concentrations indicates sufficiently intensive degradation of oil hydrocarbons. Against the background of absence of influence of pollutant concentration on the speed of basal respiration, increase in content of oil products led to a increase in the speed of substrate-induced respiration. This effect is most pronounced in the experimental varianrs of light sod-podzolic sandy loamy soil. The decrease in activity of substrate-induced respiration of dark-grey forest light loamy soil in 10 % is registered only in variants containing 4,94 and 6,45 g/kg of oil products. Practically in all variants of experiment the inhibitory effect of oil pollution on the growth of wheat roots was recorded. Sod-podzolic sandy loamy soil was most exposed to the negative influence of the pollutant. In other soils, phytoeffect is determinate by the residual oil content in soil. In the course of conducted researches somt interrelation between microbial potential (Cmic and Cmic/Corg ) and toxicity of oil polluted soils was found. The maximum toxicity for higher plants were identified in the sod-podzolic sandy loamy soil, with a minimum reserve of microbial carbon.

Lavina A.M., Khakimova L.R., Matniyazov R.T., Vershinina Z.R., Baymiev A.Kh.
THE OBTAINING OF THE RECOMBINANT STRAINS OF RHIZOBIA CARRYING AN EXTRA COPY OF GENES PSSA AND ROSR LABELED WITH FLUORESCENT PROTEIN GFP ^{[№ 9 ' 2017]}
The establishment of specific symbiotic relationships between rhizobia and leguminous plants is a complex process that requires the exchange of signaling intermediary molecules between both partners. The flavonoids, secreted by the roots of leguminous plants, induce rhizobium expression of nodule-forming genes — Nod-factors. Synthesis of Nod factors is necessary, but not sufficient for the morphogenesis of effective nitrogen fixing nodules. Since no less important factor in the formation of nodules is the synthesis of rhizobacteria exopolysaccharides (EPS), which play a decisive role in the symbiotic interactions of nodule bacteria with leguminous plants. In addition, in free-living cells, rhizobia EPSs are responsible for attachment to abiotic and biotic surfaces and for the formation of biofilms that ensure the adaptation of bacteria to changing environmental conditions. Currently, the formation of biofilms by rhizobia and the relationship of this process with effective nodule formation is being intensively studied. Special attention should be paid to the rosR gene, which encodes the transcriptional regulator involved in EPS biosynthesis, as well as the pssA gene responsible for initiating the process of EPS biosynthesis in rhizobia.
To study the genes responsible for the synthesis of EPS in the context of their impact on the formation of biofilms by rhizobia, in particular in artificial symbiotic systems, where colonization of plant roots plays an important role, it is necessary to visualize the interaction of nitrogen fixing bacteria with plant roots. In view of this, the aim of this work was to obtain recombinant rhizobial strains labeled with fluorescent protein GFP by the pssA and rosR genes. To do this, a genetically engineered construct containing the fluorescent protein gene GFP was used, which serves to visualize the interaction of rhizobia with plant roots. Cloning of pssA and rosR genes into plasmid pJB658GFP, transformation of rhizobium with this construct, microscopic examination of the obtained recombinant strains was carried out.
It has been shown that recombinant rhizobial strains with an additional copy of the pssA or rosR gene are characterized by a large mismatch of the cell walls, compared to wild strains, which indicates their high production of exopolysaccharides and, as a consequence, indicates an increase in the competitiveness of the strains.

Khakimova L.R., Serbaeva E.R., Lavina A.M., Vershinina Z.R., Baymiev A.Kh.
GROWTH-STIMULATING ACTIVITY OF BACTERIA RHIZOBIUM LEGUMINOSARUM, SELECTED FROM LEGUMINOUS PLANTS OF THE SOUTH URAL ^{[№ 9 ' 2017]}
One of the promising methods for the developmrntally-oriented farming is the use of PGPR microorganisms. They can act as an effective growth promoter and, as a result, plant productivity by improving mineral nutrition and protecting against phytopathogens. One of the most important and promising groups of PGPR are the nodule bacteria of the genus Rhizobium, which are widely used in agriculture as associative microsymbionts for nonleguminous economically significant cultures.
The strains R. leguminosarum LSy10 (D18), LVe13 (Inz 2008), PVu5 isolated from legumes of the South Urals were tested on the growth-stimulating activityon seeds of cucumber and physalis plants. It has been shown that the strains R. leguminosarum LSy10 (D18), LVe13 (Inz 2008), PVu5 have growth-stimulating activity and can be used as biofertilizers for physalis and cucumber in the concentration of 107cfu / ml and 105cfu / ml. In addition, it was found that all strains have antagonistic activity against the phytopathogenic fungus F. solani and have a positive effect on plant growth.
Thus, these strains can be used as biofertilizers to protect useful crops from phytopathogenic fungi, as well as to improve plant growth.

Galitsyn D.I., Vershinin V.L.
A NUMBER OF MORPHOLOGICAL PARAMETERS SPECIFIC OF SAND LIZARD (LACERTA AGILIS LINNAEUS, 1758) POPULATIONS IN THE MIDDLE URALS ^{[№ 6 ' 2014]}
The materials about morphological features of sand lizard populations in Sverdlovsk region are considered. The sand lizards head pholidosis variations detailed analysis in the Urals populations is carried. A number of L. agilis morphological parameters with it in southern West Siberia affinity as well as character of Kamensk area L. agilis local population are noticed.

Neustroeva N.S., Vershinin V.L.
SKELETAL DEVIATIONS OF FINGERLINGS OF TAILLESS AMPHIBIAN IN AN URBANIZING ^{[№ 4 ' 2011]}
Using the method of illumination of the soft tissues the authors studied a sample of fingerlings of three species of tailless amphibian (Rana ridibunda, R. arvalis, R. temporaria) from populations living in urban and suburban areas of big industrial center — the city of Yekaterinburg, on the subject of deviant skeletal forms. It was established range, frequency and differences due to species affiliation and degree of anthropogenic transformation of the environment. It is shown that the most informative, well reflecting the gradient of anthropogenic transformation of the environment was kind of moor frog, which has high plasticity and variability of the adaptive strategy.

^{[№ 12 (app.1) ' 2005]}

^{[№ 7 ' 2005]}

Vershinina L.V.
SCIENTIFIC PRECONDITIONS OF A VALUABLE PROBLEMATICS IN I.KANTA'S PHILOSOPHY ^{[№ 1 ' 2004]}
The problem of revealing of philosophical - aesthetic preconditions of a valuable problematics are represented with theoretical and practical interest for pedagogics as the decision of a problem of familiarizing of pupils to values is connected to development of valuable consciousness of the teacher. Time border of becoming of scientific representations about value as central concept of axiology is the end XXYIII century. In the article the analysis is carried out on I. Kant s positions about the estimated and valuable attitude of a man to the world, which represent scientific interest for pedagogics in aspect of revealing of essence and the contents of valuable consciousness of the teacher.

Vershilin I.B.
OSSIAN: POET-MYTH CREATED BY MC PHERSON. ^{[№ 6 ' 2002]}