Trutyaeva A.C.
APPLICATION OF THE BIOLUMINESCENCE METHOD TO ASSESS THE QUALITY FRESH WATER ^{[№ 5 ' 2017]}
Evaluation biotoxicity of fresh water Sakmara by bioluminescence, as well as examine the contents of heavy metals in the water. The evaluation was conducted in 3 locations: the beach “Volna” Sakmarskaya TPP effects used genetic engineered luminescent strain Esherichia coli K12 TG1, constitutively expressing luxCDABE-genes of natural marine microorganism Photobacterium leiongnathi 54D10, production of HBO “Immunotech” (Moscow, Russia) in a lyophilized state under the trade name “Ekolyum”. It has been established that emergency extinguishing the glow has a water sample from Sakmara river in the village of Tatar Kargala that can be interpreted as a manifestation of severe acute toxicity of the sample. Water samples from all other sections of the river are low degree of toxicity, close to control values. In the study of heavy metals found in the water reservoirs of the three investigated iron does not exceed standards for copper maximum admissible concentration has been exceeded several times in all three samples, with the highest concentration observed in the village of Tatar Kargala. The amount of zinc exceeds MPC only in samples taken from the beach “Volna” and the site near the village of Tatar Kargala. Thus, the results of this work indicated that by using a rapid method based on fluorescent bacterial test systems “Ekolyum” can evaluate the toxicity and pollution of waters studied.