Nikiyan H.N., Tatlybaeva E.B.
ATOMIC FORCE MICROSCOPYUSING IN IDENTIFICATION OF SPECIFICALLY MARKED MOLECULES AND MICROORGANISM CELLS ^{[№ 13 ' 2015]}
Identification of biological molecules and microorganisms is an important problem of the microbiology which is traditionally solved by means of the marked antibodies. However in most cases the corresponding methods are based on detection of a significant amount of the interacting molecules and don't allow to find out their distribution on a surface. One of possible solutions consists in use of a modern method of atomic force microscopy. In current work the specific complexes formedbynanodimensionallabels, containing colloidal gold or amorphous carbon on a surface of bacterial cages of S. aureus and on a surface of plastic microwell immunological plateswere found and studied by means of atomic force microscopy. Morphological characteristics and the map of distribution of the revealed complexes on the studied surfaces were established. In experiments with tags sensitivity of a method was defined and comparison with the enzyme-linked immunoassay was carried out. The received results indicate high sensitivity of the offered method and certain advantages of carbon labels in comparison with gold labels, because of their easy detection and unambiguity in identification on the received images. Identification of microorganisms in model two-component biological systems was carried out and approaches to marking of bacterial cages in complex biological mixes was developed for the directed their allocation and the subsequent studying. Thus, use of a atomic force microscope in combination with nanodimensional carbon and gold labels, allows to realize a new way of identification of single molecules and microorganisms in complex biological mixes.

Nikiyan H.N., Tatlybaeva E.B.
ACHIEVEMENTS AND DEVELOPMENT PROSPECTS OF ATOMIC FORCE MICROSCOPY IN MICROBIOLOGY ^{[№ 6 ' 2014]}
In this review the possibilities of atomic force microscopy in solving of microbiological problems over the last decade are considered. The main attention is paid to the achievements and successes in the field of biodetection, virology and studying the factors of biogenic and abiogenic influence on bacterial cells. Presented results show the potential of atomic force microscopy in detection and identification of single cells and molecules, investigations of mechanical and morphological properties of bacterial cells in response to different influences, as well as the study of the intermolecular and intramolecular interactions.