Vestnik On-line
Orenburg State University may 05, 2024   RU/EN
Headings of Vestnik
Pedagogics
Psychology
Other

Search
Vak
Антиплагиат
Orcid
Viniti
ЭБС Лань
Rsl
Лицензия Creative Commons

2010, № 12



Dmitryukova M.Yu., Baimiev A.Kh., Rakhmankulova Z.F. INFLUENCE OF EXPRESSION OF GENE OF SOY LEGHEMOGLOBIN ON ANTIOXIDANT SYSTEM OF TRANSGENOSIS TOBACCO-PLANTFor studying of the influence of synthesis of soy leghemoglobin A on antioxidant system of a plant tissue we created of transgenosis tobacco-plant (Nicotiana tabacum, family Solanaceae) of brand Samsun. The content of mevalonic dialdehyde, the level of catalase and guaiacolperoxidase activity was measured for studying of antioxidant status of tissues. It was revealed that transgenosis tobacco-plants accumulated smaller dry biomass and were characterized with reduced level of lipid peroxidation and guaiacolperoxidase activity compared to control non-transgenosis plants.Key words: leghemoglobin, mevalonic dialdehyde, guaiacolperoxidase.

Download
References:

1. Verma D.P.S., Bal A.K. Intracellular site of synthesis and localization of leghemoglobin in root nodules / / Proc. Natl. Acad. Sci. USA. 1976. Vol. 73. № 11. Pp. 3843-3847.

2. Becaena M., Moran JF, Iturbe-Ormaexte I., Gogorcena Y., Escuredo Structure and function of leghemoglobins / / An. Estac.Exp.Aula Dei (Zaragoza) .1994. Vol. 21. № 3. Pp. 203-208.

3. Kosmachevsky OV Topunov AF Hemoglobin — a variety of structures and functions, "Applied Biochemistry and Microbiology. 2009. T. 45. № 6. S. 627-653.

4. Woodland OG Plant cell and reactive oxygen species. — M.: KDU, 2007. — 140.

5. Kurganov, LN Lipid peroxidation — one of the possible components of a rapid response to stress / / GM. 2001. № 6. S. 76-78.

6. Holmberg N., Lilius G., Bailey J.E., Bulow L. Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production / / Nat. biotechnol. 1997. Vol. 15. Pp. 244-247.

7. Barata RM, Chapparro-Giraldo A., Chabregas SM, Gonzales R., Labate CA, Azevedo RA, Sarath G., Lea PJ, Silva-Filho MC Targeting of soybean leghemoglobin to tobacco chloroplasts: effects on aerobic metabolism in transgenic plants / / Plant Sci. 2000. Vol. 155. Pp. 193-202.

8. Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A. A simple and general method for transferring genes into plants / / Science. 1985. № 227. Pp. 1229-1231.

9. Heath RL, Packer L. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation / / Archives in Biochemistry and Biophysics 1968. № 125. Pp. 189-198.

10. Hodges DM, DeLong JM, Forney CF, Prange RK Improving the Thiobarbituric Acid-Reactive-Substances Assay for Estimating Lipid Peroxidation in Plant Tissues Containing Anthocyanin and Other Interfering Compounds / / Planta. 1999. Vol. 207. Pp. 604-611.

11. Korolyuk MA, Ivanova LI, Majorova IG, Tokarev VE (1988) Determination of catalase activity / laboratory work. № 1. C. 16-19.

12. Ermakov AI, Arasimovich VV, Yarosh NP, Peru Y., Lukovnikova GA, MI Ikonnikova Methods for biochemical study of plants. LA: Agropromizdat, 1987. S. 41-43.

13. Bonna AL, Chaparro-Giraldo A., Appezzato-da-Gloria B., Silva-Filho MC Ectopic expression of soybean leghemoglobin in chloroplasts impairs gibberillin biosynthesis and induces dwarfism in transgenic potato plants / / Mol. breeding. 2008.Vol. 22. Pp. 613-618.

14. Parani M., Rudrabhatla S., Myers R., Weirich H., Smith B., Leaman DW, Goldman SL Microarray analysis of nitric oxide responsive transcripts in Arabidopsis / / Plant Biotechnology Journal. 2004. Vol. 2. Pp. 359-366.

15. Gardner P.R. Nitric oxide dioxygenase function and mechanism of flavohemoglobin, hemoglobin, myoglobin and their associated reductases / / J. Inorg. Biochem. 2005. Vol. 99. Pp. 247-266.

16. Merzlyak MN Activated oxygen and oxidative processes in the membranes of plant cells: Progress in science and technology. Ser. Plant Physiol. Moscow: VINITI. 1989. T. 6. 168.

17. Shevyakova NI, Stetsenko LA, Meshcheryakov AB, Kuznetsov Vl.V. Changes in the activity of peroxidase systemin the process of stress-induced formation of CAM / / Plant Physiol. 2002. T. 49. № 5. S. 670-677.

18. Chaparro-Giraldo A., Barata RM, Chabergas SM, Azevedo RA, Silva-Filho MC Soybean leghemoglobin targeted to potato chloroplasts influences growth and development of transgenic plants / / Cell plant reports. 2000. Vol.19. Pp. 961-965.

19. Sievers G., Ronnberg M. Study of pseudoperoxidatic activity of soybean leghemoglobin and sperm whale myoglobin / / Biochim. Biophys. Acta. 1978. Vol. 533. Pp. 293-301.

20. Moreau S., Davies MJ, Mathieu C., Herouart D., Puppo A. Leghemoglobin derived radicals. Evidence for multiple protein-derived radicals and the initiations of the peribacteroid membrane damage / / J. Biol. Chem. 1996. Vol. 271. Pp. 32557-32562

21. Puppo A. Halliwell B (1988) Generation of hidroxyl radicals by soybean nodule leghaemoglobin. / / Planta Vol. 173.Pp. 405-410.


About this article

Authors: Baymiev A.H., Rahmankulova Z.F., Dmitryukova M.Yu.

Year: 2010


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

Crossref
Cyberleninka
Doi
Europeanlibrary
Googleacademy
scienceindex
worldcat
© Электронное периодическое издание: ВЕСТНИК ОГУ on-line (VESTNIK OSU on-line), ISSN on-line 1814-6465
Зарегистрировано в Федеральной службе по надзору в сфере связи, информационных технологий и массовых коммуникаций
Свидетельство о регистрации СМИ: Эл № ФС77-37678 от 29 сентября 2009 г.
Учредитель: Оренбургский государственный университет (ОГУ)
Главный редактор: С.А. Мирошников
Адрес редакции: 460018, г. Оренбург, проспект Победы, д. 13, к. 2335
Тел./факс: (3532)37-27-78 E-mail: vestnik@mail.osu.ru
1999–2024 © CIT OSU