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Manuhov I.V.
Karimov I.F., Manukhov I.V., Ivanov Y.B., Deryabin D.G. BACILLUS SUBTILIS VKPM V-10548 STRAIN AND IT APPLICATION FOR SERUM BLOOD BACTIRICIDAL ACTIVITY RESEARCH The recombinant Bacillus subtilis strain carrying plasmid with gram-negative sea microorganism Photobacterium leiognathi luxAB genes, before each introduced gram-positive bacteria ribosome-binding site is designed. This strain characterized by high gene expression level of bacterial luciferase and was deposited in the Russian National Collection of Industrial Microorganisms under № V-10548. In researching of B.subtilis VKPM V-10548 reaction in contact with different component serum blood probes the correspondence between bioluminescence suppression and bactericidal effect level mainly determined by beta-lysine activity is shown. Key words: bioluminescence, lux-genes, Bacillus subtilis, bactericidal effect, beta-lysine
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Editor-in-chief |
Sergey Aleksandrovich MIROSHNIKOV |
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