Vestnik On-line
Orenburg State University march 28, 2024   RU/EN
Headings of Vestnik
Pedagogics
Psychology
Other

Search
Vak
Антиплагиат
Orcid
Viniti
ЭБС Лань
Rsl
Лицензия Creative Commons

Nikiyan A.N.

June 2017, № 7 (207)


Davydova О.К., Nikiyan Н.N.
1,3-DIHYDROXY-4-HEXYLRESORCINOL INCREASES BACTERIAL CELLS SENSITIVITY TO DAMAGING ACTION OF CARBON NANOMATERIALS
The relevance of disinfecting of objects of the environment is defined by need of interruption of the main ways of bacterial infections transfer. To achieve this goal, non-reagent (physical) and reagent (chemical) methods are currently used. At the same time the first ones generally include ultraviolet treatment, and the second ones —treatment with oxidants or specially selected chemical compounds. However, over time, microorganisms develop protective mechanisms for such damaging effects, so it seems possible to sensitize (increase the sensitivity) bacterial cells to already known disinfectants, and to develop new disinfectants that realize previously unknown mechanisms of antibacterial activity. In presented work increase in sensitivity of bacterial cells to damaging action of carbon nanomaterials (fullerenes and carbon nanotubes) by use of 1,3-dihydroxy-4-hexylbenzene is shown. Its action on bacterial cells significantly increases their surface affinity, strengthening of the subsequent influence of carbon nanodisinfectants and facilitating the removal of aggregates of bacterial cells and the nanocarbon particles acting on them from the media to be purified. The experimental studies form a basis for development of innovative nanodisinfectants and their compositions interesting for practical use in a disinfectology, sanitation and hygiene.
Key words: bacterial cells sensitization, Escherichia coli, 1,3-dihydroxy-4-hexylbenzene, carbon nanomaterials, fullerene derivatives, carbon nanotubes, disinfection, antimicrobic action.

November 2015, № 13 (188)


Nikiyan H.N., Tatlybaeva E.B.
ATOMIC FORCE MICROSCOPYUSING IN IDENTIFICATION OF SPECIFICALLY MARKED MOLECULES AND MICROORGANISM CELLS
Identification of biological molecules and microorganisms is an important problem of the microbiology which is traditionally solved by means of the marked antibodies. However in most cases the corresponding methods are based on detection of a significant amount of the interacting molecules and don't allow to find out their distribution on a surface. One of possible solutions consists in use of a modern method of atomic force microscopy. In current work the specific complexes formedbynanodimensionallabels, containing colloidal gold or amorphous carbon on a surface of bacterial cages of S. aureus and on a surface of plastic microwell immunological plateswere found and studied by means of atomic force microscopy. Morphological characteristics and the map of distribution of the revealed complexes on the studied surfaces were established. In experiments with tags sensitivity of a method was defined and comparison with the enzyme-linked immunoassay was carried out. The received results indicate high sensitivity of the offered method and certain advantages of carbon labels in comparison with gold labels, because of their easy detection and unambiguity in identification on the received images. Identification of microorganisms in model two-component biological systems was carried out and approaches to marking of bacterial cages in complex biological mixes was developed for the directed their allocation and the subsequent studying. Thus, use of a atomic force microscope in combination with nanodimensional carbon and gold labels, allows to realize a new way of identification of single molecules and microorganisms in complex biological mixes.
Key words: molecules identification, microorganisms detection, gold and carbon labels, atomic force microscopy.

2014, № 6 (167)


Nikiyan H.N., Tatlybaeva E.B.
ACHIEVEMENTS AND DEVELOPMENT PROSPECTS OF ATOMIC FORCE MICROSCOPY IN MICROBIOLOGY
In this review the possibilities of atomic force microscopy in solving of microbiological problems over the last decade are considered. The main attention is paid to the achievements and successes in the field of biodetection, virology and studying the factors of biogenic and abiogenic influence on bacterial cells. Presented results show the potential of atomic force microscopy in detection and identification of single cells and molecules, investigations of mechanical and morphological properties of bacterial cells in response to different influences, as well as the study of the intermolecular and intramolecular interactions.
Key words: atomic force microscopy, bacterial cells, identification of molecules, detection of viral particles.

2012, № 10 (146)


Vasilchenko A.S., Yarullina D.R., Nikiyan H.N., Teslya A.V.
MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF THE BACTERIA BACILLUS CEREUS AT VARIOUS STAGES OF THE LIFE CYCLE
Using atomic force microscopy (AFM) the life cycle of spore-forming Gram-positive organisms Bacillus cereus IP 5832 was investigated. The morphological and mechanical changes of single bacterial cells by their long-term culturing were described. It is shown decreasing the number of viable cells at the 5 day of cultivation and no viable cells in bacterial population at 15 day. In this case, the presence of spores which are morphologically differentiated forms of resting cells was revealed by AFM.
Key words: atomic force microscopy, life cycle, mechanical properties, bacterial cells, long-term culturing.

2011, № 12


Vasilchenko A.S., Nikiyan H.N., Deryabin D.G.
THE USE OF AGAROSE FILMS AS MODEL STRUCTURES FOR ATOMIC FORCE MICROSCOPE PREPARATION FOR FURTHER STUDY OF THE ELASTIC PROPERTIES OF BACTERIAL CELLS
Elastic properties of agarose films depending on the concentration and relative humidity of the samples environment were quantitatively characterized using atomic force microscopy in force spectroscopy mode. The results are discussed in the context of agarose films use as a test reference material to prepare an atomic force microscope for the further study of the mechanical properties of bacterial cells.
Key words: atomic force microscopy, polymer films, agarose, mechanical properties, bacterial cells

2005, № 1


Davydova O.K., Nikiyan A.N., Deryabin D.G.
FORMING OF WELL-ORDERED PERMOLECULAR STRUCTURES OF DNA IN WATER SOLUTIONS IN PRESENCE OF ALKYLRESORCINOLS
The forming of well-ordered line permolecular structures of high-polymer DNA, happened in water solutions in presence of low-molecular ligands from the group of alkylresorcinols is dangerous. Consistent stages of similar process, beginning with creation of micellesimolar structures on detached filaments of DNA and finishing with their integration in cable structures, surrounded by single alkylresorcinol "cover", are visualized with using of atomic, power and scanning electron microscopy. The model of interaction between these molecules are suggested in this article on the base of represented results, and also earlier got data about changes of physical and chemical characteristics od DNA and alkylresorcinols.

2003, № 1


Salikova S.P., Stadnikov A.A., Nikiyan A.N.
ATOMIC-POWER MICROSCOPE AS A NEW OPPORTUNITY OF CARDIOMYOCYTES STUDYING
The article considers the atomic-power microscope work main principles and its application results when studying rats' myocardium cells surface. Atomic-power method advantages are mentioned. Atomic-power method application allows not only to visualize the cell surface but to gain information on its different properties.

2001, № 4


Letuta S.N., Nikiyan A.N., Davidova O.K.
RODOMINE STAINS AND DNA INTERACTION.
The article deals with the investigation of rodamine stains and DNA interaction by means of spectrophotometric methods and probe scanning microscopy. If was shown that rodamines interact with macromolecules by cooperative fixation with the external past of polymeric chain. As a result organic nanostructures (supramolecular systems) are formed on the polymer surface. The stain molecule quantify is estimated. The distribution of generated structures along the double DNA spiral and their fusion in larger conglomerates are investigated. The authors draw a conclusion about double spiral screening by means of stains and the nucleinic acids inactivation resulting in violations of their biological functions.


Editor-in-chief
Sergey Aleksandrovich
MIROSHNIKOV

Crossref
Cyberleninka
Doi
Europeanlibrary
Googleacademy
scienceindex
worldcat
© Электронное периодическое издание: ВЕСТНИК ОГУ on-line (VESTNIK OSU on-line), ISSN on-line 1814-6465
Зарегистрировано в Федеральной службе по надзору в сфере связи, информационных технологий и массовых коммуникаций
Свидетельство о регистрации СМИ: Эл № ФС77-37678 от 29 сентября 2009 г.
Учредитель: Оренбургский государственный университет (ОГУ)
Главный редактор: С.А. Мирошников
Адрес редакции: 460018, г. Оренбург, проспект Победы, д. 13, к. 2335
Тел./факс: (3532)37-27-78 E-mail: vestnik@mail.osu.ru
1999–2024 © CIT OSU